Biomaterial Database

Stage-specific induction of sister-chromatid exchanges in utero. 1984

N P Singh, and A Turturro, and R W Hart

In order to correlate the induction of sister-chromatid exchanges (SCE) to biological endpoints, and elucidate aspects of this relationships, 7,12-dimethylbenz[a]anthracene (DMBA) and methyl methanesulfonate (MMS), chemicals with different biological actions at different stages in development, have been evaluated for their ability to induce SCE at different gestational ages in the Sprague-Dawley rat. Transplacental exposure to these agents was accomplished by a recently developed intraperitoneal infusion technique to replenish metabolically degraded 5-bromo-2'-deoxyuridine used for SCE visualization. Maternal bone marrow and whole fetal tissue, fetal liver and fetal brain were compared. Day-9 embryo was found to be very sensitive to the effects of both agents, with the ability to induce SCE declining in development in whole fetus and fetal organs. The embryotoxic effects of the agents seem to be ones best correlated with the capacity of the agents to induce SCE. Also, fetal liver is more sensitive than fetal brain to the effects of DMBA compared with MMS, suggesting fetal metabolic activation may have occurred. Measurement of the amount of radiolabelled DMBA reaching the fetal tissue used to estimate SCE indicates that the amount of chemical reaching the fetus does not account for the increased sensitivity, especially at Day 9. Some factor(s) in development, such as differentiation stage, rather than the fetal accessibility to chemical, seem to be important in the induction of SCE in utero.

UI	MeSH Term	Description	Entries
D008741	Methyl Methanesulfonate	An alkylating agent in cancer therapy that may also act as a mutagen by interfering with and causing damage to DNA.	Methylmethane Sulfonate,Dimethylsulfonate,Mesilate, Methyl,Methyl Mesylate,Methyl Methylenesulfonate,Methylmesilate,Mesylate, Methyl,Methanesulfonate, Methyl,Methyl Mesilate
D009024	Morphogenesis	The development of anatomical structures to create the form of a single- or multi-cell organism. Morphogenesis provides form changes of a part, parts, or the whole organism.
D009152	Mutagenicity Tests	Tests of chemical substances and physical agents for mutagenic potential. They include microbial, insect, mammalian cell, and whole animal tests.	Genetic Toxicity Tests,Genotoxicity Tests,Mutagen Screening,Tests, Genetic Toxicity,Toxicity Tests, Genetic,Genetic Toxicity Test,Genotoxicity Test,Mutagen Screenings,Mutagenicity Test,Screening, Mutagen,Screenings, Mutagen,Test, Genotoxicity,Tests, Genotoxicity,Toxicity Test, Genetic
D011247	Pregnancy	The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH.	Gestation,Pregnancies
D003434	Crossing Over, Genetic	The reciprocal exchange of segments at corresponding positions along pairs of homologous CHROMOSOMES by symmetrical breakage and crosswise rejoining forming cross-over sites (HOLLIDAY JUNCTIONS) that are resolved during CHROMOSOME SEGREGATION. Crossing-over typically occurs during MEIOSIS but it may also occur in the absence of meiosis, for example, with bacterial chromosomes, organelle chromosomes, or somatic cell nuclear chromosomes.	Crossing Over,Crossing-Over, Genetic,Crossing Overs,Genetic Crossing Over,Genetic Crossing-Over
D005260	Female		Females
D005333	Fetus	The unborn young of a viviparous mammal, in the postembryonic period, after the major structures have been outlined. In humans, the unborn young from the end of the eighth week after CONCEPTION until BIRTH, as distinguished from the earlier EMBRYO, MAMMALIAN.	Fetal Structures,Fetal Tissue,Fetuses,Mummified Fetus,Retained Fetus,Fetal Structure,Fetal Tissues,Fetus, Mummified,Fetus, Retained,Structure, Fetal,Structures, Fetal,Tissue, Fetal,Tissues, Fetal
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D001551	Benz(a)Anthracenes	Four fused benzyl rings with three linear and one angular, that can be viewed as a benzyl-phenanthrenes. Compare with NAPHTHACENES which are four linear rings.	Benz(b)Phenanthrenes,Naphthanthracenes