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In this study various C27 sterols with a 3-oxo-4-ene structure were incubated with adrenal cortex mitochondrial preparations. (22R)-22-Hydroxy-4-cholesten-3-one and (20R,22R)-20,22-dihydroxy-4-cholesten-3-one were found to be converted into progesterone. This suggests the existence of a pathway for adrenal progesterone formation analogous to the normal 3 beta-hydroxy-5-ene pathways. (20S)-20-Hydroxy-4-cholesten-3-one was hydroxylated at C25. 4-Cholesten-3-one, 25-hydroxy-4-cholesten-3-one and (22S)-22-hydroxy-4-cholesten-3-one were not converted to a measurable extent. With 3-oxo-4-ene C27 sterols as substrates, the cholesterol side-chain cleaving enzyme system seems to require the presence of a 22R-hydroxyl group in the substrate. The clinical relevance of these observations is discussed.
H J Degenhart, and
G J Alsema, and
J Hoogerbrugge, and
B G Wolthers, and
R Kaptein
January 1992,
Biotechnology advances,
H J Degenhart, and
G J Alsema, and
J Hoogerbrugge, and
B G Wolthers, and
R Kaptein
January 1992,
Yao xue xue bao = Acta pharmaceutica Sinica,
H J Degenhart, and
G J Alsema, and
J Hoogerbrugge, and
B G Wolthers, and
R Kaptein
February 1985,
The Journal of clinical investigation,
H J Degenhart, and
G J Alsema, and
J Hoogerbrugge, and
B G Wolthers, and
R Kaptein
January 1985,
Journal of basic microbiology,
H J Degenhart, and
G J Alsema, and
J Hoogerbrugge, and
B G Wolthers, and
R Kaptein
December 1982,
Steroids,
H J Degenhart, and
G J Alsema, and
J Hoogerbrugge, and
B G Wolthers, and
R Kaptein
September 2009,
Acta crystallographica. Section E, Structure reports online,
H J Degenhart, and
G J Alsema, and
J Hoogerbrugge, and
B G Wolthers, and
R Kaptein
May 1985,
Journal of steroid biochemistry,
H J Degenhart, and
G J Alsema, and
J Hoogerbrugge, and
B G Wolthers, and
R Kaptein
October 1998,
The Journal of steroid biochemistry and molecular biology,
H J Degenhart, and
G J Alsema, and
J Hoogerbrugge, and
B G Wolthers, and
R Kaptein
January 1996,
Ukrainskii biokhimicheskii zhurnal (1978),
H J Degenhart, and
G J Alsema, and
J Hoogerbrugge, and
B G Wolthers, and
R Kaptein
January 2013,
Acta crystallographica. Section E, Structure reports online,
