Biomaterial Database

The genome of simian hemorrhagic fever virus. 1984

J L Sagripanti

Techniques are described for preparing intact Simian Hemorrhagic Fever (SHF) virus RNA. SHF RNA extracted by proteinase K digestion in the presence of sodium dodecyl sulphate (SDS) has a sedimentation coefficient of 49 S compared with a reference figure of 47 S for Sindbis RNA. Purified SHF RNA in cesium sulphate gradient has a buoyant density of 1.63 g/ml similar to that of Sindbis RNA. This result leads to the conclusion that SHF RNA is single stranded. This is supported by results on RNAse sensitivity and analyses on sucrose gradients under different ionic strength conditions. Electrophoresis analyses on both hydroxymethylmercuric acid or formaldehyde containing gels, gave a value of about 5.5 X 10(6) daltons for the molecular weight of SHF RNA. No evidence of subunit structure was found. From these results, we conclude that the SHF virus genome is a single continuous chain of about 15,000 ribonucleotides.

UI	MeSH Term	Description	Entries
D008970	Molecular Weight	The sum of the weight of all the atoms in a molecule.	Molecular Weights,Weight, Molecular,Weights, Molecular
D008992	Monkey Diseases	Diseases of Old World and New World monkeys. This term includes diseases of baboons but not of chimpanzees or gorillas (	Disease, Monkey,Diseases, Monkey,Monkey Disease
D009691	Nucleic Acid Denaturation	Disruption of the secondary structure of nucleic acids by heat, extreme pH or chemical treatment. Double strand DNA is "melted" by dissociation of the non-covalent hydrogen bonds and hydrophobic interactions. Denatured DNA appears to be a single-stranded flexible structure. The effects of denaturation on RNA are similar though less pronounced and largely reversible.	DNA Denaturation,DNA Melting,RNA Denaturation,Acid Denaturation, Nucleic,Denaturation, DNA,Denaturation, Nucleic Acid,Denaturation, RNA,Nucleic Acid Denaturations
D002499	Centrifugation, Density Gradient	Separation of particles according to density by employing a gradient of varying densities. At equilibrium each particle settles in the gradient at a point equal to its density. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)	Centrifugations, Density Gradient,Density Gradient Centrifugation,Density Gradient Centrifugations,Gradient Centrifugation, Density,Gradient Centrifugations, Density
D006482	Hemorrhagic Fevers, Viral	A group of viral diseases of diverse etiology but having many similar clinical characteristics; increased capillary permeability, leukopenia, and thrombocytopenia are common to all. Hemorrhagic fevers are characterized by sudden onset, fever, headache, generalized myalgia, backache, conjunctivitis, and severe prostration, followed by various hemorrhagic symptoms. Hemorrhagic fever with kidney involvement is HEMORRHAGIC FEVER WITH RENAL SYNDROME.	Fever, Viral Hemorrhagic,Fevers, Viral Hemorrhagic,Hemorrhagic Fever, Viral,Viral Hemorrhagic Fever,Viral Hemorrhagic Fevers
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D012367	RNA, Viral	Ribonucleic acid that makes up the genetic material of viruses.	Viral RNA
D014035	Togaviridae	A family of RNA viruses, mainly arboviruses, consisting of two genera: ALPHAVIRUS (group A arboviruses), and RUBIVIRUS. Virions are spherical, 60-70 nm in diameter, with a lipoprotein envelope tightly applied to the icosahedral nucleocapsid.	Togaviruses,Togavirus