-transparent.png){kind=logo}
Enhanced NADPH-dependent LPO in rat liver postmitochondrial supernatants in vitro due to depletion of GSH by treatment with phorone (diisopropylidene acetone) in vivo was inhibited by Fe2+-chelating agents (desferrioxamine, DETAPAC), but not by scavengers of .O2-, H2O2, singlet oxygen or .OH-radicals, indicating that a perferryl ion (Fe2+ . O2) is needed as an initiating factor. In vivo, rats treated with phorone (250 mg/kg i.p.) exhaled 1.4 times as much ethane within 4 h as compared to controls. Pretreatment with FeSO4 resulted in a 6.9-fold enhancement of LPO as compared to Fe2+-pretreated controls. Our results indicate that GSH-depletion results in a strong enhancement of NADPH-dependent LPO also in vivo, provided that an initiating factor is present.
M Younes, and
M Albrecht, and
C P Siegers
June 2000,
Biological trace element research,
M Younes, and
M Albrecht, and
C P Siegers
January 1987,
General pharmacology,
M Younes, and
M Albrecht, and
C P Siegers
December 1989,
Biochemistry international,
M Younes, and
M Albrecht, and
C P Siegers
January 2007,
Molecular and cellular biochemistry,
M Younes, and
M Albrecht, and
C P Siegers
April 1980,
Biochimica et biophysica acta,
M Younes, and
M Albrecht, and
C P Siegers
March 1990,
Archives of biochemistry and biophysics,
M Younes, and
M Albrecht, and
C P Siegers
June 1997,
Research communications in molecular pathology and pharmacology,
M Younes, and
M Albrecht, and
C P Siegers
July 1979,
The Journal of biological chemistry,
M Younes, and
M Albrecht, and
C P Siegers
March 1989,
Archives of biochemistry and biophysics,
M Younes, and
M Albrecht, and
C P Siegers
May 1980,
Molecular pharmacology,
