Biomaterial Database

Domain-structure of large monomeric alanyl-tRNA synthetase from Bombyx mori: evidence of a single catalytic domain. 1984

K Nishio, and M Kawakami

Alanyl-tRNA synthetase of 115K dalton from Bombyx mori was cleaved into two fragments of 68K and 47K dalton with trypsin. The 47K fragment was active in aminoacylation of tRNA, whereas the 68K fragment inactive. The 47K and 68K fragments were located at the N- and C- terminal sides, respectively, in the intact enzyme. When the enzyme binds alanine specific tRNA, the tryptic digestion is inhibited. The Km value of the 47K fragment for tRNA was about 16-fold higher than that (1.4 microM) of the intact enzyme. The molecular activities of the 47K fragment and the intact enzyme were 2.2/sec and 16.8/sec, respectively. These results show that 1) Bombyx mori alanyl-tRNA synthetase functions in a monomeric state and 2) the C-terminal domain enhances affinity for tRNA and is responsible for full activity of aminoacylation.

UI	MeSH Term	Description	Entries
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D008970	Molecular Weight	The sum of the weight of all the atoms in a molecule.	Molecular Weights,Weight, Molecular,Weights, Molecular
D000412	Alanine-tRNA Ligase	An enzyme that activates alanine with its specific transfer RNA. EC 6.1.1.7.	Alanyl T RNA Synthetase,Ala-tRNA Ligase,Alanyl-tRNA Synthetase,Ala tRNA Ligase,Alanine tRNA Ligase,Alanyl tRNA Synthetase,Ligase, Ala-tRNA,Ligase, Alanine-tRNA,Synthetase, Alanyl-tRNA
D000604	Amino Acyl-tRNA Synthetases	A subclass of enzymes that aminoacylate AMINO ACID-SPECIFIC TRANSFER RNA with their corresponding AMINO ACIDS.	Amino Acyl T RNA Synthetases,Amino Acyl-tRNA Ligases,Aminoacyl Transfer RNA Synthetase,Aminoacyl-tRNA Synthetase,Transfer RNA Synthetase,tRNA Synthetase,Acyl-tRNA Ligases, Amino,Acyl-tRNA Synthetases, Amino,Amino Acyl tRNA Ligases,Amino Acyl tRNA Synthetases,Aminoacyl tRNA Synthetase,Ligases, Amino Acyl-tRNA,RNA Synthetase, Transfer,Synthetase, Aminoacyl-tRNA,Synthetase, Transfer RNA,Synthetase, tRNA,Synthetases, Amino Acyl-tRNA
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D001665	Binding Sites	The parts of a macromolecule that directly participate in its specific combination with another molecule.	Combining Site,Binding Site,Combining Sites,Site, Binding,Site, Combining,Sites, Binding,Sites, Combining
D012831	Bombyx	A genus of silkworm MOTHS in the family Bombycidae of the order LEPIDOPTERA. The family contains a single species, Bombyx mori from the Greek for silkworm + mulberry tree (on which it feeds). A native of Asia, it is sometimes reared in this country. It has long been raised for its SILK and after centuries of domestication it probably does not exist in nature. It is used extensively in experimental GENETICS. (From Borror et al., An Introduction to the Study of Insects, 4th ed, p519)	Bombyx mori,Silkmoths,Silkworms,Silkmoth,Silkworm,Bombyx morus,Bombyxs,mori, Bombyx