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We describe a totally enzymatic method for determining serum triglycerides adapted for a System Olli 3000 analyser. In this procedure triglycerides are determined by measuring free glycerol enzymatically after hydrolysis of a sample with lipase and esterase. The method utilizes two standards for calibration, includes a blank correction and requires 20 microliter of serum. Forty serum samples can be analysed in about 30 min. The procedure is linear up to a concentration of 8 mmol/l of triglycerides. The precision and sensitivity of the method are good. A comparison of this method with another enzymatic method gave the correlation coefficient 0.988 and the regression line y = 1.02x + 0.02 (n = 95).
R Puukka, and
H Jokela, and
M Puukka
January 1980,
The Journal of automatic chemistry,
R Puukka, and
H Jokela, and
M Puukka
October 1981,
Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie,
R Puukka, and
H Jokela, and
M Puukka
January 1977,
Annales de biologie clinique,
R Puukka, and
H Jokela, and
M Puukka
January 1967,
Annales de biologie clinique,
R Puukka, and
H Jokela, and
M Puukka
January 1977,
Annales de biologie clinique,
R Puukka, and
H Jokela, and
M Puukka
February 1978,
Clinical chemistry,
R Puukka, and
H Jokela, and
M Puukka
January 1996,
The Journal of automatic chemistry,
R Puukka, and
H Jokela, and
M Puukka
March 1975,
Clinical chemistry,
R Puukka, and
H Jokela, and
M Puukka
October 1977,
Der Internist,
R Puukka, and
H Jokela, and
M Puukka
January 1980,
Zeitschrift fur medizinische Laboratoriumsdiagnostik,
