BIOMAT Database Logo BIOMAT Database Logo

Each element of the Drosophila tRNAArg gene split promoter directs transcription in Xenopus oocytes. 1983

S Sharp, and T Dingermann, and J Schaack, and J A Sharp, and D J Burke, and E M DeRobertis, and D Söll

The intragenic control regions of a eukaryotic tRNA gene have been examined by transcribing mutant forms of a Drosophila tRNAArg gene either by injection into the nucleus of Xenopus oocytes or in extracts prepared from isolated oocyte nuclei. These experiments demonstrate that the selection of the transcription initiation site is a complex mechanism that involves the T-control region, the D-control region, and sequences 5' adjacent to the D-control region. In this study either "half" of the Drosophila tRNAArg gene promoted transcription in Xenopus oocytes. This finding supports a recent model for eukaryotic tRNA gene transcription (Dingermann et al., 1983, J. Biol. Chem. 258, 10395-10402) that proposes transcription initiation is dependent on the ability of specific DNA sequences to sequester two RNA polymerase III transcription factors.

UI MeSH Term Description Entries
D009865 Oocytes Female germ cells derived from OOGONIA and termed OOCYTES when they enter MEIOSIS. The primary oocytes begin meiosis but are arrested at the diplotene state until OVULATION at PUBERTY to give rise to haploid secondary oocytes or ova (OVUM). Ovocytes,Oocyte,Ovocyte
D009876 Operon In bacteria, a group of metabolically related genes, with a common promoter, whose transcription into a single polycistronic MESSENGER RNA is under the control of an OPERATOR REGION. Operons
D010957 Plasmids Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS. Episomes,Episome,Plasmid
D002467 Cell Nucleus Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed) Cell Nuclei,Nuclei, Cell,Nucleus, Cell
D002872 Chromosome Deletion Actual loss of portion of a chromosome. Monosomy, Partial,Partial Monosomy,Deletion, Chromosome,Deletions, Chromosome,Monosomies, Partial,Partial Monosomies
D004274 DNA, Recombinant Biologically active DNA which has been formed by the in vitro joining of segments of DNA from different sources. It includes the recombination joint or edge of a heteroduplex region where two recombining DNA molecules are connected. Genes, Spliced,Recombinant DNA,Spliced Gene,Recombinant DNA Research,Recombination Joint,DNA Research, Recombinant,Gene, Spliced,Joint, Recombination,Research, Recombinant DNA,Spliced Genes
D004330 Drosophila A genus of small, two-winged flies containing approximately 900 described species. These organisms are the most extensively studied of all genera from the standpoint of genetics and cytology. Fruit Fly, Drosophila,Drosophila Fruit Flies,Drosophila Fruit Fly,Drosophilas,Flies, Drosophila Fruit,Fly, Drosophila Fruit,Fruit Flies, Drosophila
D005260 Female Females
D005796 Genes A category of nucleic acid sequences that function as units of heredity and which code for the basic instructions for the development, reproduction, and maintenance of organisms. Cistron,Gene,Genetic Materials,Cistrons,Genetic Material,Material, Genetic,Materials, Genetic
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia

Related Publications

S Sharp, and T Dingermann, and J Schaack, and J A Sharp, and D J Burke, and E M DeRobertis, and D Söll
Transcription of a Drosophila heat shock gene is heat-induced in Xenopus oocytes.
March 1982, Proceedings of the National Academy of Sciences of the United States of America,
S Sharp, and T Dingermann, and J Schaack, and J A Sharp, and D J Burke, and E M DeRobertis, and D Söll
A 14 bp promoter element directs the testis specificity of the Drosophila beta 2 tubulin gene.
May 1989, The EMBO journal,
S Sharp, and T Dingermann, and J Schaack, and J A Sharp, and D J Burke, and E M DeRobertis, and D Söll
A novel promoter element, photoreceptor conserved element II, directs photoreceptor-specific expression of nocturnin in Xenopus laevis.
May 2001, The Journal of biological chemistry,
S Sharp, and T Dingermann, and J Schaack, and J A Sharp, and D J Burke, and E M DeRobertis, and D Söll
A cis regulatory element in the TAPNAC promoter directs tapetal gene expression.
January 2011, Plant molecular biology,
S Sharp, and T Dingermann, and J Schaack, and J A Sharp, and D J Burke, and E M DeRobertis, and D Söll
Premature termination of tubulin gene transcription in Xenopus oocytes is due to promoter-dependent disruption of elongation.
December 1993, Molecular and cellular biology,
S Sharp, and T Dingermann, and J Schaack, and J A Sharp, and D J Burke, and E M DeRobertis, and D Söll
Spacer regulation of Xenopus ribosomal gene transcription: competition in oocytes.
December 1983, Cell,
S Sharp, and T Dingermann, and J Schaack, and J A Sharp, and D J Burke, and E M DeRobertis, and D Söll
Transcription of a Drosophila tRNAArg gene in yeast extract: 5'-flanking sequence dependence for transcription in a heterologous system.
April 1985, Nucleic acids research,
S Sharp, and T Dingermann, and J Schaack, and J A Sharp, and D J Burke, and E M DeRobertis, and D Söll
Intron sequences modulate feather keratin gene transcription in Xenopus oocytes.
August 1986, Nucleic acids research,
S Sharp, and T Dingermann, and J Schaack, and J A Sharp, and D J Burke, and E M DeRobertis, and D Söll
Upstream sequences required for transcription of the TFIIIA gene in Xenopus oocytes.
May 1988, Nucleic acids research,
S Sharp, and T Dingermann, and J Schaack, and J A Sharp, and D J Burke, and E M DeRobertis, and D Söll
Promoter control of translation in Xenopus oocytes.
February 1995, Nucleic acids research,
Copied contents to your clipboard!