BIOMAT Database Logo BIOMAT Database Logo

Transcription of a Drosophila heat shock gene is heat-induced in Xenopus oocytes. 1982

R Voellmy, and D Rungger

Xenopus cells, like many other eukaryotic cells, respond to heat treatments by increasing the rate of synthesis of a few characteristic proteins, the heat shock proteins. Because of the generality of this response, it seemed possible to examine the expression of isolated heat shock genes in a heterologous system. Phage 122 DNA, containing two identical genes coding for the Drosophila 70,000-dalton heat shock protein (hsp70 genes), was microinjected into Xenopus oocyte nuclei. The Drosophila hsp70 genes are transcribed efficiently in heat-treated oocytes (35-37 degrees C) to give RNA of the correct size and sequence content. Transcription is sensitive to low levels of alpha-amanitin and therefore is carried out by RNA polymerase II. At normal temperatures (20-28 degrees C) essentially no Drosophila-specific RNA is formed. The isolated insert fragment of phage 122 also gives RNA of correct length in heat-treated oocytes which hybridizes to the coding segment of Drosophila hsp70 genes only. At normal temperatures, however, its rate of transcription is variable and only RNA heterogeneous in size is formed.

UI MeSH Term Description Entries
D008970 Molecular Weight The sum of the weight of all the atoms in a molecule. Molecular Weights,Weight, Molecular,Weights, Molecular
D011506 Proteins Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein. Gene Products, Protein,Gene Proteins,Protein,Protein Gene Products,Proteins, Gene
D004331 Drosophila melanogaster A species of fruit fly frequently used in genetics because of the large size of its chromosomes. D. melanogaster,Drosophila melanogasters,melanogaster, Drosophila
D005786 Gene Expression Regulation Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation. Gene Action Regulation,Regulation of Gene Expression,Expression Regulation, Gene,Regulation, Gene Action,Regulation, Gene Expression
D005796 Genes A category of nucleic acid sequences that function as units of heredity and which code for the basic instructions for the development, reproduction, and maintenance of organisms. Cistron,Gene,Genetic Materials,Cistrons,Genetic Material,Material, Genetic,Materials, Genetic
D006358 Hot Temperature Presence of warmth or heat or a temperature notably higher than an accustomed norm. Heat,Hot Temperatures,Temperature, Hot,Temperatures, Hot
D006360 Heat-Shock Proteins Proteins which are synthesized in eukaryotic organisms and bacteria in response to hyperthermia and other environmental stresses. They increase thermal tolerance and perform functions essential to cell survival under these conditions. Stress Protein,Stress Proteins,Heat-Shock Protein,Heat Shock Protein,Heat Shock Proteins,Protein, Stress
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D014982 Xenopus laevis The commonest and widest ranging species of the clawed "frog" (Xenopus) in Africa. This species is used extensively in research. There is now a significant population in California derived from escaped laboratory animals. Platanna,X. laevis,Platannas,X. laevi

Related Publications

R Voellmy, and D Rungger
Do Xenopus oocytes have a heat shock response?
February 1987, Developmental biology,
R Voellmy, and D Rungger
Expression of a Drosophila heat-shock protein in Xenopus oocytes: conserved and divergent regulatory signals.
January 1982, The EMBO journal,
R Voellmy, and D Rungger
Distinct stress-inducible and developmentally regulated heat shock transcription factors in Xenopus oocytes.
January 1997, Developmental biology,
R Voellmy, and D Rungger
The heat-shock response in Xenopus oocytes is controlled at the translational level.
July 1982, Cell,
R Voellmy, and D Rungger
Each element of the Drosophila tRNAArg gene split promoter directs transcription in Xenopus oocytes.
December 1983, Nucleic acids research,
R Voellmy, and D Rungger
Drosophila gene related to the major heat shock-induced gene is transcribed at normal temperatures and not induced by heat shock.
January 1982, Proceedings of the National Academy of Sciences of the United States of America,
R Voellmy, and D Rungger
A DNA damage-responsive Drosophila melanogaster gene is also induced by heat shock.
December 1986, Molecular and cellular biology,
R Voellmy, and D Rungger
Integration, transcription, and control of a Drosophila heat shock gene in mouse cells.
November 1981, Proceedings of the National Academy of Sciences of the United States of America,
R Voellmy, and D Rungger
Response to heat shock of gene 1, a Drosophila melanogaster small heat shock gene, is developmentally regulated.
May 1991, Molecular & general genetics : MGG,
R Voellmy, and D Rungger
Heat-induced degradation of PER and TIM in Drosophila bearing a conditional allele of the heat shock transcription factor gene.
July 1999, Chronobiology international,
Copied contents to your clipboard!