-transparent.png){kind=logo}
A mixed-sequence synthetic oligonucleotide probe was used to isolate a clone containing the gene encoding the alpha subunit of bacterial luciferase from Vibrio harveyi and part of the gene coding for the beta subunit. DNA sequence analysis has allowed us to determine that the genes are closely linked on the bacterial chromosome and transcribed in the same direction. Comparison of the sequences in the regions preceding the two structural genes has revealed considerable homology and has identified sites that may be involved in the expression of the genes. Identification of a clone from a clone bank of total genomic DNA from this organism shows that mixed probes can be successfully used to isolate a gene of interest from any bacterium provided some protein sequence for the gene product is available.
D H Cohn, and
R C Ogden, and
J N Abelson, and
T O Baldwin, and
K H Nealson, and
M I Simon, and
A J Mileham
July 1984,
Biochemistry,
D H Cohn, and
R C Ogden, and
J N Abelson, and
T O Baldwin, and
K H Nealson, and
M I Simon, and
A J Mileham
January 1986,
Archives of microbiology,
D H Cohn, and
R C Ogden, and
J N Abelson, and
T O Baldwin, and
K H Nealson, and
M I Simon, and
A J Mileham
November 1982,
Science (New York, N.Y.),
D H Cohn, and
R C Ogden, and
J N Abelson, and
T O Baldwin, and
K H Nealson, and
M I Simon, and
A J Mileham
October 2006,
Archives of biochemistry and biophysics,
D H Cohn, and
R C Ogden, and
J N Abelson, and
T O Baldwin, and
K H Nealson, and
M I Simon, and
A J Mileham
January 1985,
The Journal of biological chemistry,
D H Cohn, and
R C Ogden, and
J N Abelson, and
T O Baldwin, and
K H Nealson, and
M I Simon, and
A J Mileham
June 1981,
Journal of bacteriology,
D H Cohn, and
R C Ogden, and
J N Abelson, and
T O Baldwin, and
K H Nealson, and
M I Simon, and
A J Mileham
January 1999,
Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica,
D H Cohn, and
R C Ogden, and
J N Abelson, and
T O Baldwin, and
K H Nealson, and
M I Simon, and
A J Mileham
January 1989,
Biochimica et biophysica acta,
D H Cohn, and
R C Ogden, and
J N Abelson, and
T O Baldwin, and
K H Nealson, and
M I Simon, and
A J Mileham
March 2002,
Biochemistry,
D H Cohn, and
R C Ogden, and
J N Abelson, and
T O Baldwin, and
K H Nealson, and
M I Simon, and
A J Mileham
January 2003,
Biochemistry,
