Biomaterial Database

Control of sugar utilization in the oral bacteria Streptococcus salivarius and Streptococcus sanguis by the phosphoenolpyruvate: glucose phosphotransferase system. 1983

C Vadeboncoeur, and G Bourgeau, and D Mayrand, and L Trahan

Three different Strep. salivarius (G2, G5 and G29) and two Strep. sanguis (GS3 and GS12) mutants affected in the phosphoenolpyruvate: glucose phosphotransferase system were selected on agar plates containing lactose and 2-deoxyglucose. All 5 were defective in a membrane-bound component of the transport system and grew less rapidly than the parent strain in 5 mM glucose-containing medium. Mutants G2 and G29 grew poorly in the presence of 5 mM mannose. Growth on mixed substrates revealed that the mutants and wild-type parents behaved differently. Wild-type strains in medium containing glucose plus another sugar (lactose, galactose, melibiose, raffinose or trehalose for Strep. salivarius and lactose, galactose or trehalose for Strep. sanguis) always exhausted most of the glucose before utilizing the other sugar. The mutants used the second sugar concurrently or preferentially to glucose. In medium containing glucose plus fructose or mannose, the wild types consumed both sugars concurrently whereas the mutants utilized the second sugar before glucose. Mutants G2 and G5 were insensitive to repression by fructose and released glucose into the medium when grown in the presence of 0.4 per cent lactose. Mutant G5 also released galactose. Sugar release was not detected with the wild types. The Strep. salivarius mutants contained normal levels of glucokinase and beta-galactosidase but G5 was almost totally devoid of galactokinase activity after growth on lactose. On galactose, the activity was restored. It seems that the phosphoenolpyruvate: glucose phosphotransferase system is involved in the regulation of sugar utilization in these two streptococci.

UI	MeSH Term	Description	Entries
D009005	Monosaccharides	Single chain carbohydrates that are the most basic units of CARBOHYDRATES. They are typically colorless crystalline substances with a sweet taste and have the same general formula CnH2nOn.	Monosaccharide,Simple Sugar,Simple Sugars,Sugar, Simple,Sugars, Simple
D009055	Mouth	The oval-shaped oral cavity located at the apex of the digestive tract and consisting of two parts: the vestibule and the oral cavity proper.	Oral Cavity,Cavitas Oris,Cavitas oris propria,Mouth Cavity Proper,Oral Cavity Proper,Vestibule Oris,Vestibule of the Mouth,Cavity, Oral
D009154	Mutation	Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.	Mutations
D010731	Phosphoenolpyruvate Sugar Phosphotransferase System	The bacterial sugar phosphotransferase system (PTS) that catalyzes the transfer of the phosphoryl group from phosphoenolpyruvate to its sugar substrates (the PTS sugars) concomitant with the translocation of these sugars across the bacterial membrane. The phosphorylation of a given sugar requires four proteins, two general proteins, Enzyme I and HPr and a pair of sugar-specific proteins designated as the Enzyme II complex. The PTS has also been implicated in the induction of synthesis of some catabolic enzyme systems required for the utilization of sugars that are not substrates of the PTS as well as the regulation of the activity of ADENYLYL CYCLASES. EC 2.7.1.-.	Phosphoenolpyruvate Hexose Phosphotransferases,Phosphoenolpyruvate-Glycose Phosphotransferase System,Hexose Phosphotransferases, Phosphoenolpyruvate,Phosphoenolpyruvate Glycose Phosphotransferase System,Phosphotransferase System, Phosphoenolpyruvate-Glycose,Phosphotransferases, Phosphoenolpyruvate Hexose,System, Phosphoenolpyruvate-Glycose Phosphotransferase
D005947	Glucose	A primary source of energy for living organisms. It is naturally occurring and is found in fruits and other parts of plants in its free state. It is used therapeutically in fluid and nutrient replacement.	Dextrose,Anhydrous Dextrose,D-Glucose,Glucose Monohydrate,Glucose, (DL)-Isomer,Glucose, (alpha-D)-Isomer,Glucose, (beta-D)-Isomer,D Glucose,Dextrose, Anhydrous,Monohydrate, Glucose
D013291	Streptococcus	A genus of gram-positive, coccoid bacteria whose organisms occur in pairs or chains. No endospores are produced. Many species exist as commensals or parasites on man or animals with some being highly pathogenic. A few species are saprophytes and occur in the natural environment.
D013298	Streptococcus sanguis	A gram-positive organism found in dental plaque, in blood, on heart valves in subacute endocarditis, and infrequently in saliva and throat specimens. L-forms are associated with recurrent aphthous stomatitis.	Streptococcus sanguinis