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We have previously isolated a 500 bp-long cDNA clone, NO9-15, which is derived from a nuclear transcript originating from the heat shock locus 2-48B of Drosophila hydei (Peters et al. 1982). Sequence analysis shows that this clone carries 4 complete copies and 1 partial copy of a 115 bp repeat unit. The repeats are closely homologous with a maximal sequence divergence of about 10%. The sequence does not contain an open reading frame. The genomic organization of heat shock locus 2-48B, as probed with the cloned cDNA sequence NO9-15, is highly polymorphic. Four different allelic arrangements have been found in different inbred strains. A number of genomic clones isolated from region 2-48B, both in phage lambda and in cosmid vectors, all differ in length, mainly due to varying numbers of the NO9-15 repeat unit. These differences are found primarily in the proximal region of the locus. The transcribed region of these clones includes the distal sequence flanking the NO9-15 repeat as well as the NO9-15 repeat itself. An oligo A stretch was found between the distal flanking sequence and the NO9-15 repeat region.
F P Peters, and
N H Lubsen, and
U Walldorf, and
R J Moormann, and
B Hovemann
July 1983,
European journal of cell biology,
F P Peters, and
N H Lubsen, and
U Walldorf, and
R J Moormann, and
B Hovemann
April 1987,
Nucleic acids research,
F P Peters, and
N H Lubsen, and
U Walldorf, and
R J Moormann, and
B Hovemann
March 1978,
Chromosoma,
F P Peters, and
N H Lubsen, and
U Walldorf, and
R J Moormann, and
B Hovemann
November 1985,
Molecular and cellular biology,
F P Peters, and
N H Lubsen, and
U Walldorf, and
R J Moormann, and
B Hovemann
August 1978,
European journal of biochemistry,
F P Peters, and
N H Lubsen, and
U Walldorf, and
R J Moormann, and
B Hovemann
January 1971,
Genetica,
F P Peters, and
N H Lubsen, and
U Walldorf, and
R J Moormann, and
B Hovemann
September 1976,
Nature,
F P Peters, and
N H Lubsen, and
U Walldorf, and
R J Moormann, and
B Hovemann
December 1975,
Molecular & general genetics : MGG,
F P Peters, and
N H Lubsen, and
U Walldorf, and
R J Moormann, and
B Hovemann
September 1983,
Proceedings of the National Academy of Sciences of the United States of America,
F P Peters, and
N H Lubsen, and
U Walldorf, and
R J Moormann, and
B Hovemann
March 1979,
Biochimica et biophysica acta,
