-transparent.png){kind=logo}
Poly(A)-positive mRNA extracted from tonsillar mononuclear cells stimulated with phytohemagglutinin-M and 12-o-tetradecanoyl phorbol 13-acetate was successfully translated into biologically active interleukin 2 (IL-2) in Xenopus laevis oocytes, and secreted into the incubation medium. In control experiments, the extract of oocytes injected with either poly(A)-negative RNA or buffer did not show any IL-2 activity. By sucrose density gradient centrifugation analysis, IL-2 mRNA was found as a single peak corresponding to a sedimentation coefficient of 10-11S.
Related Publications
T Hirano, and
T Sudo, and
T Teranishi, and
K Fujimoto, and
M Iizuka, and
S Kobayashi, and
K Onoue
October 1980,
Journal of virology,
T Hirano, and
T Sudo, and
T Teranishi, and
K Fujimoto, and
M Iizuka, and
S Kobayashi, and
K Onoue
November 1982,
Biochemical and biophysical research communications,
T Hirano, and
T Sudo, and
T Teranishi, and
K Fujimoto, and
M Iizuka, and
S Kobayashi, and
K Onoue
January 1981,
Methods in enzymology,
T Hirano, and
T Sudo, and
T Teranishi, and
K Fujimoto, and
M Iizuka, and
S Kobayashi, and
K Onoue
April 1985,
Immunological investigations,
T Hirano, and
T Sudo, and
T Teranishi, and
K Fujimoto, and
M Iizuka, and
S Kobayashi, and
K Onoue
October 1978,
FEBS letters,
T Hirano, and
T Sudo, and
T Teranishi, and
K Fujimoto, and
M Iizuka, and
S Kobayashi, and
K Onoue
January 1995,
Methods in molecular biology (Clifton, N.J.),
T Hirano, and
T Sudo, and
T Teranishi, and
K Fujimoto, and
M Iizuka, and
S Kobayashi, and
K Onoue
May 1981,
Biochemical and biophysical research communications,
T Hirano, and
T Sudo, and
T Teranishi, and
K Fujimoto, and
M Iizuka, and
S Kobayashi, and
K Onoue
May 1978,
Biochemical and biophysical research communications,
T Hirano, and
T Sudo, and
T Teranishi, and
K Fujimoto, and
M Iizuka, and
S Kobayashi, and
K Onoue
December 1984,
FEBS letters,
T Hirano, and
T Sudo, and
T Teranishi, and
K Fujimoto, and
M Iizuka, and
S Kobayashi, and
K Onoue
December 1976,
The Journal of clinical investigation,
