Seasonal plasma progesterone, androstenedione, estrone, and 17 beta-estradiol concentrations in the female Rana esculenta were determined by radioimmunoassay during the 1979 and 1981 seasons. Plasma levels of these steroids were highest just before the first ovulatory wave in spring and lowest after the breeding season. In the 1979 season (during the 1981 season hormones were not assayed in January, November, and December) progesterone, androstenedione, and estradiol levels showed another peak in November-December. During the breeding months, i.e., late March to late June, progesterone, androstenedione, and estradiol levels showed intermittent ups and downs corresponding roughly to the ovulatory waves. In addition, during the breeding season progesterone and androstenedione levels had a higher average in frogs with "ripe" ovaries than in those with "spent" ovaries. Relationships between seasonal steroid levels and ovarian activity are briefly discussed.