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The rapid one-step gradient centrifugation procedure for simultaneous isolation of granulocytic and mononuclear leukocytes from human blood: biological, physical and chemical bases. 1983

Y H Thong, and J M Currell, and R L Rodwell

When anti-coagulated blood is layered over a ficoll-hypaque gradient of special chemical composition and optimal density of 1.114 gm per ml, the leukocytes resolve into two distinct bands at the interface after centrifugation at 400G for 20-30 minutes. These two bands are usually at least 0.9 mm apart, and can be easily removed separately. The top band consists of predominantly mononuclear cells, while granulocytes predominate in the lower band. This simple, rapid method for the isolation of blood leukocytes of high purity is now available as Mono-Poly Resolving Medium (Flow Laboratories, Virginia), but the curious phenomenon on which it is based has yet to be satisfactorily explained. In this paper, we report on preliminary observations which indicate that both the erythrocytes and chemical composition of the separation medium crucial roles in the process.

UI MeSH Term Description Entries
D009000 Monocytes Large, phagocytic mononuclear leukocytes produced in the vertebrate BONE MARROW and released into the BLOOD; contain a large, oval or somewhat indented nucleus surrounded by voluminous cytoplasm and numerous organelles. Monocyte
D002499 Centrifugation, Density Gradient Separation of particles according to density by employing a gradient of varying densities. At equilibrium each particle settles in the gradient at a point equal to its density. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) Centrifugations, Density Gradient,Density Gradient Centrifugation,Density Gradient Centrifugations,Gradient Centrifugation, Density,Gradient Centrifugations, Density
D006098 Granulocytes Leukocytes with abundant granules in the cytoplasm. They are divided into three groups according to the staining properties of the granules: neutrophilic, eosinophilic, and basophilic. Mature granulocytes are the NEUTROPHILS; EOSINOPHILS; and BASOPHILS. Granulocyte
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D066298 In Vitro Techniques Methods to study reactions or processes taking place in an artificial environment outside the living organism. In Vitro Test,In Vitro Testing,In Vitro Tests,In Vitro as Topic,In Vitro,In Vitro Technique,In Vitro Testings,Technique, In Vitro,Techniques, In Vitro,Test, In Vitro,Testing, In Vitro,Testings, In Vitro,Tests, In Vitro,Vitro Testing, In

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