Biomaterial Database

Preparation and calibration of a reference preparation for human serum proteins. 1984

E J Hamilton, and W Tostowaryk, and J A Caron, and A Godard, and B W Griffiths

A reference preparation for human serum proteins (RPHSP) has been developed to aid in the standardization of a wide variety of protein analytes commonly tested by immunodiagnostic procedures. The U.S. national RPHSP was used to cross-calibrate our reference serum by single radial immunodiffusion for the analytes alpha 1-acid glycoprotein, alpha 1-antitrypsin, alpha 2-macroglobulin, ceruloplasmin, C3, C4, haptoglobin, transferrin, IgA, IgG, IgM and by Laurell "rocket" assay for albumin. The statistical evaluation of the cross-calibration study indicates high precision of estimates of the specific proteins assigned to the Canadian RPHSP relative to those of the U.S. reference preparation. Protein concentrations were assigned in both WHO international units (when available) and mass units.

UI	MeSH Term	Description	Entries
D007123	Immunoelectrophoresis, Two-Dimensional	Immunoelectrophoresis in which a second electrophoretic transport is performed on the initially separated antigen fragments into an antibody-containing medium in a direction perpendicular to the first electrophoresis.	Immunoelectrophoresis, Crossed,Immunoelectrophoresis, 2-D,Immunoelectrophoresis, 2D,2-D Immunoelectrophoresis,2D Immunoelectrophoresis,Crossed Immunoelectrophoresis,Immunoelectrophoresis, 2 D,Immunoelectrophoresis, Two Dimensional,Two-Dimensional Immunoelectrophoresis
D008297	Male		Males
D012015	Reference Standards	A basis of value established for the measure of quantity, weight, extent or quality, e.g. weight standards, standard solutions, methods, techniques, and procedures used in diagnosis and therapy.	Standard Preparations,Standards, Reference,Preparations, Standard,Standardization,Standards,Preparation, Standard,Reference Standard,Standard Preparation,Standard, Reference
D001797	Blood Protein Electrophoresis	Electrophoresis applied to BLOOD PROTEINS.	Hemoglobin Electrophoresis,Electrophoresis, Blood Protein,Electrophoresis, Hemoglobin,Protein Electrophoresis, Blood
D001798	Blood Proteins	Proteins that are present in blood serum, including SERUM ALBUMIN; BLOOD COAGULATION FACTORS; and many other types of proteins.	Blood Protein,Plasma Protein,Plasma Proteins,Serum Protein,Serum Proteins,Protein, Blood,Protein, Plasma,Protein, Serum,Proteins, Blood,Proteins, Plasma,Proteins, Serum
D002170	Canada	The largest country in North America, comprising 10 provinces and three territories. Its capital is Ottawa.
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000328	Adult	A person having attained full growth or maturity. Adults are of 19 through 44 years of age. For a person between 19 and 24 years of age, YOUNG ADULT is available.	Adults
D012709	Serum Albumin	A major protein in the BLOOD. It is important in maintaining the colloidal osmotic pressure and transporting large organic molecules.	Plasma Albumin,Albumin, Serum
D014481	United States	A country in NORTH AMERICA between CANADA and MEXICO.