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[L-glutaminase-asparaginase biosynthesis by a Pseudomonas boreopolis culture dependent on cultivation conditions]. 1978

A G Chigaleĭchik, and B D Vinogradov, and V S Orlova, and S S Rylkin

The activity of deamidases of L-glutamine and L-asparagine of Pseudomonas boreopolis 526 was found to depend on the conditions of cultivation. The activity of L-glutaminase-asparaginase II (EC 3.5.1.38) decreased with an increase of the specific growth rate in the conditions of periodic cultivation and increased in the conditions of continuous cultivation. The activity of this enzyme was manifested mainly in the conditions of continuous cultivation at a rate of 0.1--0.2 hr-1 and a final nitrogen concentration of 0.006--0.025 g per litre of the medium. If the concentration of nitrogen was increased in these conditions, biosynthesis of L-glutaminase-asparaginase I was stimulated while the activity of L-glutaminase-asparaginase II was not detected at all.

UI MeSH Term Description Entries
D011549 Pseudomonas A genus of gram-negative, aerobic, rod-shaped bacteria widely distributed in nature. Some species are pathogenic for humans, animals, and plants. Chryseomonas,Pseudomona,Flavimonas
D003470 Culture Media Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN. Media, Culture
D004789 Enzyme Activation Conversion of an inactive form of an enzyme to one possessing metabolic activity. It includes 1, activation by ions (activators); 2, activation by cofactors (coenzymes); and 3, conversion of an enzyme precursor (proenzyme or zymogen) to an active enzyme. Activation, Enzyme,Activations, Enzyme,Enzyme Activations
D005973 Glutamine A non-essential amino acid present abundantly throughout the body and is involved in many metabolic processes. It is synthesized from GLUTAMIC ACID and AMMONIA. It is the principal carrier of NITROGEN in the body and is an important energy source for many cells. D-Glutamine,L-Glutamine,D Glutamine,L Glutamine
D000581 Amidohydrolases Any member of the class of enzymes that catalyze the cleavage of amide bonds and result in the addition of water to the resulting molecules. Amidases,Amidohydrolase
D001216 Asparagine A non-essential amino acid that is involved in the metabolic control of cell functions in nerve and brain tissue. It is biosynthesized from ASPARTIC ACID and AMMONIA by asparagine synthetase. (From Concise Encyclopedia Biochemistry and Molecular Biology, 3rd ed) L-Asparagine
D013997 Time Factors Elements of limited time intervals, contributing to particular results or situations. Time Series,Factor, Time,Time Factor

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