-transparent.png){kind=logo}
We describe an automated, kinetic nephelometric method for fibronectin on the Multistat III F/LS Centrifugal Analyzer (Instrumentation Laboratory Inc., Lexington, MA 02173). Antiserum is diluted with polyethylene glycol. Calibrators and samples are prediluted with potassium phosphate buffer (10 mmol/L, pH 7.0) containing 8.5 g of NaCl per litre. Intensity (I) is read at 5 and 180 s and the resulting delta I plotted against concentration. A non-linear least squares curve fitting and interpolation of results is carried out automatically. Three controls, with values of between 190-370 gave coefficients of variation between 4 and 7 percent, and the sensitivity of the method is to 25 mg/L.
B W Steele, and
L P Hills, and
R L Hellman, and
F Civantos, and
J Kent
September 1980,
Clinical chemistry,
B W Steele, and
L P Hills, and
R L Hellman, and
F Civantos, and
J Kent
August 1987,
Clinical chemistry,
B W Steele, and
L P Hills, and
R L Hellman, and
F Civantos, and
J Kent
December 1983,
Clinical biochemistry,
B W Steele, and
L P Hills, and
R L Hellman, and
F Civantos, and
J Kent
January 1980,
Haemostasis,
B W Steele, and
L P Hills, and
R L Hellman, and
F Civantos, and
J Kent
January 1984,
Thrombosis research,
B W Steele, and
L P Hills, and
R L Hellman, and
F Civantos, and
J Kent
January 1979,
Clinical chemistry,
B W Steele, and
L P Hills, and
R L Hellman, and
F Civantos, and
J Kent
July 1988,
Analytical biochemistry,
B W Steele, and
L P Hills, and
R L Hellman, and
F Civantos, and
J Kent
January 1982,
Annales de biologie clinique,
B W Steele, and
L P Hills, and
R L Hellman, and
F Civantos, and
J Kent
November 1987,
Clinical chemistry,
B W Steele, and
L P Hills, and
R L Hellman, and
F Civantos, and
J Kent
July 1986,
Clinical chemistry,
