Biomaterial Database

Antigens and allergens from the common house dust mite Dermatophagoides pteronyssinus. Part I. Demonstration of multiple allergens by immunochemical and biologic analyses. 1984

S Krilis, and B A Baldo, and R Sutton, and A Basten

The complexity of a highly allergenic water-soluble extract of the common house dust mite Dermatophagoides pteronyssinus has been investigated by use of the preparative fractionation techniques of ultrafiltration, gel permeation chromatography, and isoelectric focusing, and the allergenic properties of the resultant fractions are assessed by skin prick tests, RAST, and a histamine-release assay. In addition, two analytical approaches were used to identify allergens-radioimmunoelectrophoresis and a new technique for detecting IgE-binding proteins separated by isoelectric focusing and transferred onto a nitrocellulose membrane by transverse electrophoresis. These various techniques for allergen detection revealed differences in the number and distribution of allergens in the crude mite extract. The data obtained are consistent with the conclusion that allergenic activity of D. pteronyssinus extracts is due to the existence of a number of antigenically distinct allergens and not due to a single, major allergen.

UI	MeSH Term	Description	Entries
D007525	Isoelectric Focusing	Electrophoresis in which a pH gradient is established in a gel medium and proteins migrate until they reach the site (or focus) at which the pH is equal to their isoelectric point.	Electrofocusing,Focusing, Isoelectric
D008925	Mites	Any arthropod of the subclass ACARI except the TICKS. They are minute animals related to the spiders, usually having transparent or semitransparent bodies. They may be parasitic on humans and domestic animals, producing various irritations of the skin (MITE INFESTATIONS). Many mite species are important to human and veterinary medicine as both parasite and vector. Mites also infest plants.	Acarus,Mite
D011852	Radioallergosorbent Test	An in vitro allergen radioimmunoassay in which allergens are coupled to an immunosorbent. The coupled allergens bind the IgE in the sera of patients which in turn binds radioisotope-labeled anti-IMMUNOGLOBULIN E antibodies.	Radioimmunosorbent Assay of Allergens,Allergens Radioimmunosorbent Assay,Allergens Radioimmunosorbent Assays,Radioallergosorbent Tests,Test, Radioallergosorbent,Tests, Radioallergosorbent
D002850	Chromatography, Gel	Chromatography on non-ionic gels without regard to the mechanism of solute discrimination.	Chromatography, Exclusion,Chromatography, Gel Permeation,Chromatography, Molecular Sieve,Gel Filtration,Gel Filtration Chromatography,Chromatography, Size Exclusion,Exclusion Chromatography,Gel Chromatography,Gel Permeation Chromatography,Molecular Sieve Chromatography,Chromatography, Gel Filtration,Exclusion Chromatography, Size,Filtration Chromatography, Gel,Filtration, Gel,Sieve Chromatography, Molecular,Size Exclusion Chromatography
D004391	Dust	Earth or other matter in fine, dry particles. (Random House Unabridged Dictionary, 2d ed)	House Dust,Housedust,Dust, House
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000485	Allergens	Antigen-type substances that produce immediate hypersensitivity (HYPERSENSITIVITY, IMMEDIATE).	Allergen
D000941	Antigens	Substances that are recognized by the immune system and induce an immune reaction.	Antigen
D014462	Ultrafiltration	The separation of particles from a suspension by passage through a filter with very fine pores. In ultrafiltration the separation is accomplished by convective transport; in DIALYSIS separation relies instead upon differential diffusion. Ultrafiltration occurs naturally and is a laboratory procedure. Artificial ultrafiltration of the blood is referred to as HEMOFILTRATION or HEMODIAFILTRATION (if combined with HEMODIALYSIS).