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We have developed a competitive enzyme immunoassay for prolactin in human serum. Serum samples were incubated at 4 degrees C for 48 h with anti-prolactin antibodies and prolactin labeled with beta-D-galactosidase. The antibody-bound form of labeled prolactin was separated from the unbound form by a method based on the thiol-disulfide interchange reaction. By measuring the enzyme activity, serum prolactin could be determined. Sensitivity of the assay was 2.5 ng/ml, and the assay was as sensitive as radioimmunoassay. There was a good correlation between the values obtained by the enzyme immunoassay and those obtained by a radioimmunoassay (r = 0.98, slope = 1.26, y-intercept = - 12.8 ng/ml).
K Ishikawa, and
O Narita, and
H Noguchi, and
K Kato
January 1990,
Clinical chemistry,
K Ishikawa, and
O Narita, and
H Noguchi, and
K Kato
February 1980,
Acta endocrinologica,
K Ishikawa, and
O Narita, and
H Noguchi, and
K Kato
February 1995,
Journal of immunoassay,
K Ishikawa, and
O Narita, and
H Noguchi, and
K Kato
March 1987,
Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao,
K Ishikawa, and
O Narita, and
H Noguchi, and
K Kato
February 1965,
Science (New York, N.Y.),
K Ishikawa, and
O Narita, and
H Noguchi, and
K Kato
September 1995,
Analytical chemistry,
K Ishikawa, and
O Narita, and
H Noguchi, and
K Kato
May 1980,
Clinical chemistry,
K Ishikawa, and
O Narita, and
H Noguchi, and
K Kato
April 1993,
Clinical chemistry,
K Ishikawa, and
O Narita, and
H Noguchi, and
K Kato
January 1984,
Journal of applied biochemistry,
K Ishikawa, and
O Narita, and
H Noguchi, and
K Kato
March 1998,
Clinica chimica acta; international journal of clinical chemistry,
