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Immunochemical determination of human tear lysozyme (muramidase) in keratoconjunctivitis sicca. 1982

P T Janssen, and O P van Bijsterveld

Radial immunodiffusion was tested as an alternative for the agar diffusion assay for lysozyme in tear fluid. The influence of interactions between lysozyme and agarose or filter paper, used for sample collection, on the results of the immunodiffusion test was investigated. In 100 tear samples from 50 healthy volunteers and average lysozyme concentration of 1.9 g/l was found. Lysozyme values obtained by both methods in the tear fluid of a group of 78 volunteers, ranging from healthy individuals to severe keratoconjunctivitis sicca patients were compared. A lower limit of normal at 1.1 g/l was found by the radial immunodiffusion assay.

UI MeSH Term Description Entries
D007637 Keratoconjunctivitis Simultaneous inflammation of the cornea and conjunctiva. Keratoconjunctivitides
D009113 Muramidase A basic enzyme that is present in saliva, tears, egg white, and many animal fluids. It functions as an antibacterial agent. The enzyme catalyzes the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. EC 3.2.1.17. Lysozyme,Leftose,N-Acetylmuramide Glycanhydrolase,Glycanhydrolase, N-Acetylmuramide,N Acetylmuramide Glycanhydrolase
D004058 Diffusion The tendency of a gas or solute to pass from a point of higher pressure or concentration to a point of lower pressure or concentration and to distribute itself throughout the available space. Diffusion, especially FACILITATED DIFFUSION, is a major mechanism of BIOLOGICAL TRANSPORT. Diffusions
D005374 Filtration A process of separating particulate matter from a fluid, such as air or a liquid, by passing the fluid carrier through a medium that will not pass the particulates. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) Filtrations
D005779 Immunodiffusion Technique involving the diffusion of antigen or antibody through a semisolid medium, usually agar or agarose gel, with the result being a precipitin reaction. Gel Diffusion Tests,Diffusion Test, Gel,Diffusion Tests, Gel,Gel Diffusion Test,Immunodiffusions,Test, Gel Diffusion,Tests, Gel Diffusion
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D012685 Sepharose Agarose,Sepharose 4B,Sepharose C1 4B,4B, Sepharose C1,C1 4B, Sepharose
D013666 Tears The fluid secreted by the lacrimal glands. This fluid moistens the CONJUNCTIVA and CORNEA. Meibomian Lipids,Meibum,Lipid, Meibomian,Meibomian Lipid,Tear

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