Biomaterial Database

Hydrolysis and metabolism of N-benzoyl-L-tyrosyl-p-aminobenzoic acid in normal and pancreatic duct-ligated animals. 1978

C Yamato, and K Kinoshita

Hydrolysis and metabolism of N-benzoyl-L-tyrosyl-p-aminobenzoic acid (Bz-ty-PABA), a synthetic peptide used for the assessment of exocrine pancreatic function, were studied in normal and pancreatic duct-ligated rats and guinea pigs. Bz-ty-PABA was specifically cleaved by chymotrypsin to N-benzoyl-L-tyrosine and p-aminobenzoic acid (PABA) in mucosal homogenates of the intestine. Both of the resultant products were rapidly absorbed and excreted in the urine after oral administration of two kinds of radioactive Bz-ty-PABA (Bz[14C] and PABA[14C]) to animals. The absorption rate was correlated to the intestinal chymotrypsin activity obtained from in vitro studies. Benzoic acid and p-acetaminobenzoic acid were predominant in the urine after dosages of Bz-ty-PABA with Bz[14C] and PABA[14C], respectively, in both species. Additionally, a small amount of unchanged form of Bz-ty-PABA was excreted in the urine and bile without being cleaved by chymotrypsin. These results and the study using intravenous injections suggest that the absorption of this intact peptide may be almost 10% of the dose.

UI	MeSH Term	Description	Entries
D007422	Intestines	The section of the alimentary canal from the STOMACH to the ANAL CANAL. It includes the LARGE INTESTINE and SMALL INTESTINE.	Intestine
D008026	Ligation	Application of a ligature to tie a vessel or strangulate a part.	Ligature,Ligations,Ligatures
D008297	Male		Males
D010183	Pancreatic Ducts	Ducts that collect PANCREATIC JUICE from the PANCREAS and supply it to the DUODENUM.	Duct of Santorini,Duct of Wirsung,Duodenal Papilla, Minor,Wirsung's Duct,Accessory Pancreatic Duct,Accessory Pancreatic Duct of Santorini,Main Pancreatic Duct,Santorini's Duct,Accessory Pancreatic Ducts,Duct, Accessory Pancreatic,Duct, Main Pancreatic,Duct, Pancreatic,Duct, Santorini's,Duct, Wirsung's,Ducts, Pancreatic,Main Pancreatic Ducts,Minor Duodenal Papilla,Minor Duodenal Papillas,Pancreatic Duct,Pancreatic Duct, Accessory,Pancreatic Duct, Main,Pancreatic Ducts, Accessory,Papilla, Minor Duodenal,Santorini Duct,Wirsung Duct,Wirsungs Duct
D002918	Chymotrypsin	A serine endopeptidase secreted by the pancreas as its zymogen, CHYMOTRYPSINOGEN and carried in the pancreatic juice to the duodenum where it is activated by TRYPSIN. It selectively cleaves aromatic amino acids on the carboxyl side.	Alpha-Chymotrypsin Choay,Alphacutanée,Avazyme
D004064	Digestive System	A group of organs stretching from the MOUTH to the ANUS, serving to breakdown foods, assimilate nutrients, and eliminate waste. In humans, the digestive system includes the GASTROINTESTINAL TRACT and the accessory glands (LIVER; BILIARY TRACT; PANCREAS).	Ailmentary System,Alimentary System
D006168	Guinea Pigs	A common name used for the genus Cavia. The most common species is Cavia porcellus which is the domesticated guinea pig used for pets and biomedical research.	Cavia,Cavia porcellus,Guinea Pig,Pig, Guinea,Pigs, Guinea
D006868	Hydrolysis	The process of cleaving a chemical compound by the addition of a molecule of water.
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D001646	Bile	An emulsifying agent produced in the LIVER and secreted into the DUODENUM. Its composition includes BILE ACIDS AND SALTS; CHOLESTEROL; and ELECTROLYTES. It aids DIGESTION of fats in the duodenum.	Biliary Sludge,Sludge, Biliary