Biomaterial Database

Clearance kinetics and fate of mouse IgA immune complexes prepared with monomeric or dimeric IgA. 1983

A Rifai, and M Mannik

To determine the pathophysiologic mechanism(s) involved in experimental IgA nephropathy, the clearance kinetics and tissue distribution of soluble IgA immune complexes in mice were investigated. Purified radiolabeled dimeric (dIgA) and monomeric (mIgA) IgA antidinitrophenyl, obtained from MOPC-315, were covalently cross-linked with a bivalent affinity-labeling antigen, bis-2,4-dinitrophenyl pimelic ester. After i.v. injection, heavy polymers (greater than 1.2 X 10(6) m.w.) were rapidly removed from circulation. Analysis of circulating intermediate-latticed complexes by gradient polyacrylamide gel electrophoresis indicated that polymers with a minimal composition of four dIgA or eight mIgA were required for rapid elimination. The dIgA and mIgA complexes with lattices smaller than this critical size were removed at slower rates (yielding a t1/2 of 35 min for complexes with dIgA and a t1/2 of 60 min for complexes with mIgA). Tissue distribution of both dIgA and mIgA immune complexes was similar. The liver was the major organ involved in uptake of IgA immune complexes with an insignificant amount in the bile. Heavy polymers of dIgA or mIgA were predominantly localized in the hepatic nonparenchymal cells.

UI	MeSH Term	Description	Entries
D007070	Immunoglobulin A	Represents 15-20% of the human serum immunoglobulins, mostly as the 4-chain polymer in humans or dimer in other mammals. Secretory IgA (IMMUNOGLOBULIN A, SECRETORY) is the main immunoglobulin in secretions.	IgA,IgA Antibody,IgA1,IgA2,Antibody, IgA
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D008099	Liver	A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.	Livers
D008805	Mice, Inbred A	An inbred strain of mouse that is widely used in IMMUNOLOGY studies and cancer research.	Mouse, Inbred A,Inbred A Mice,Inbred A Mouse
D008807	Mice, Inbred BALB C	An inbred strain of mouse that is widely used in IMMUNOLOGY studies and cancer research.	BALB C Mice, Inbred,BALB C Mouse, Inbred,Inbred BALB C Mice,Inbred BALB C Mouse,Mice, BALB C,Mouse, BALB C,Mouse, Inbred BALB C,BALB C Mice,BALB C Mouse
D003432	Cross-Linking Reagents	Reagents with two reactive groups, usually at opposite ends of the molecule, that are capable of reacting with and thereby forming bridges between side chains of amino acids in proteins; the locations of naturally reactive areas within proteins can thereby be identified; may also be used for other macromolecules, like glycoproteins, nucleic acids, or other.	Bifunctional Reagent,Bifunctional Reagents,Cross Linking Reagent,Crosslinking Reagent,Cross Linking Reagents,Crosslinking Reagents,Linking Reagent, Cross,Linking Reagents, Cross,Reagent, Bifunctional,Reagent, Cross Linking,Reagent, Crosslinking,Reagents, Bifunctional,Reagents, Cross Linking,Reagents, Cross-Linking,Reagents, Crosslinking
D004064	Digestive System	A group of organs stretching from the MOUTH to the ANUS, serving to breakdown foods, assimilate nutrients, and eliminate waste. In humans, the digestive system includes the GASTROINTESTINAL TRACT and the accessory glands (LIVER; BILIARY TRACT; PANCREAS).	Ailmentary System,Alimentary System
D005260	Female		Females
D005704	Gallbladder	A storage reservoir for BILE secretion. Gallbladder allows the delivery of bile acids at a high concentration and in a controlled manner, via the CYSTIC DUCT to the DUODENUM, for degradation of dietary lipid.	Gallbladders
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia