-transparent.png){kind=logo}
The isolated hepatocyte preparation has become an increasingly popular system for studying xenobiotic metabolism, and there is a need for methods of determining metabolic activity. Described here is a simple and sensitive radiometric assay for barbiturate N-demethylase activity in the isolated hepatocyte suspension. The demethylation of 1,3-dimethylphenobarbital is determined by measuring the formation of its only significant metabolite, N-methylphenobarbital. The reaction is a typical P-450-mediated dealkylation.
Related Publications
R T Abraham, and
M E Sauers, and
M A Zemaitis, and
J D Alvin
December 1981,
Biochemical pharmacology,
R T Abraham, and
M E Sauers, and
M A Zemaitis, and
J D Alvin
November 1982,
Biochimica et biophysica acta,
R T Abraham, and
M E Sauers, and
M A Zemaitis, and
J D Alvin
January 1992,
Drug metabolism and disposition: the biological fate of chemicals,
R T Abraham, and
M E Sauers, and
M A Zemaitis, and
J D Alvin
March 1998,
Toxicology letters,
R T Abraham, and
M E Sauers, and
M A Zemaitis, and
J D Alvin
November 1978,
Naunyn-Schmiedeberg's archives of pharmacology,
R T Abraham, and
M E Sauers, and
M A Zemaitis, and
J D Alvin
January 1992,
Toxicology in vitro : an international journal published in association with BIBRA,
R T Abraham, and
M E Sauers, and
M A Zemaitis, and
J D Alvin
August 1986,
Pharmacological research communications,
R T Abraham, and
M E Sauers, and
M A Zemaitis, and
J D Alvin
September 1982,
Biochemical pharmacology,
R T Abraham, and
M E Sauers, and
M A Zemaitis, and
J D Alvin
September 1985,
Cancer letters,
R T Abraham, and
M E Sauers, and
M A Zemaitis, and
J D Alvin
January 1990,
Toxicology and applied pharmacology,
