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The influence of growth rate on the kinetics of phenotypic expression of 6-thioguanine resistance has been investigated by establishing 3 protocols for the maintenance of mutagenized stock cultures of human diploid fibroblasts. These protocols differed in the frequency with which subculture was performed during the phenotypic expression period. The resultant growth kinetics varied widely from continual exponential proliferation to density-inhibited growth for much of the expression period. With all of the subculture protocols, a maximum mutation frequency was reached by 9 days after exposure of confluent cultures to 6 J/m2 of 254 nm UV light. The maximum frequency was similar in all experimental groups and remained stable up to 17 days after irradiation, the last time point tested. These results indicate that continual proliferation is not required for optimal expression of 6-thioguanine resistance.
A J Grosovsky, and
J B Little
April 1978,
Mutation research,
A J Grosovsky, and
J B Little
October 1976,
Mutation research,
A J Grosovsky, and
J B Little
February 2002,
Mutation research,
A J Grosovsky, and
J B Little
July 1976,
Mutation research,
A J Grosovsky, and
J B Little
January 1992,
Environmental and molecular mutagenesis,
A J Grosovsky, and
J B Little
February 1982,
Journal of cellular physiology,
A J Grosovsky, and
J B Little
October 1975,
Experimental cell research,
A J Grosovsky, and
J B Little
October 1992,
Proceedings of the National Academy of Sciences of the United States of America,
A J Grosovsky, and
J B Little
January 1979,
Mutation research,
A J Grosovsky, and
J B Little
July 1983,
Mutation research,
