-transparent.png){kind=logo}
A method for determining blood plasma kallikrein inhibitors is suggested. It is based on inhibiting the kininogenase activity of kallikrein. The donors' dry plasma devoid of kininases, of other proteolytic enzymes and their inhibitors was used as source and reference of prekallikrein, kallikrein and high-molecular kininogen. Freshly centrifuged plasma was incubated in the presence of unithiol at 37 degrees C in amounts producing a 30-50% inhibition of the kininogenase activity of kallikrein. The values obtained reflected the activity of kallikrein inhibitors and might be of great importance in the study of the physiological role and biochemical properties of kallikrein inhibitors in different animal species, in the diagnosis and prediction of various diseases, as criterion for laboratory control over administering tissue inhibitors of proteolytic enzymes of animal origin.
M S Surovikina, and
E E Fomina
January 1975,
Laboratornoe delo,
M S Surovikina, and
E E Fomina
July 1972,
The Journal of clinical investigation,
M S Surovikina, and
E E Fomina
March 1998,
Chemical & pharmaceutical bulletin,
M S Surovikina, and
E E Fomina
January 1984,
Medycyna doswiadczalna i mikrobiologia,
M S Surovikina, and
E E Fomina
February 1999,
Bioorganic & medicinal chemistry letters,
M S Surovikina, and
E E Fomina
May 1976,
Biulleten' eksperimental'noi biologii i meditsiny,
M S Surovikina, and
E E Fomina
May 1996,
Immunopharmacology,
M S Surovikina, and
E E Fomina
August 1994,
Blood coagulation & fibrinolysis : an international journal in haemostasis and thrombosis,
M S Surovikina, and
E E Fomina
August 1985,
Biulleten' eksperimental'noi biologii i meditsiny,
M S Surovikina, and
E E Fomina
August 2006,
Biological chemistry,
