Biomaterial Database

Partial purification of a human liver sulphotransferase active towards bile salts. 1980

L Lööf, and S Hjertén

An enzyme that catalyzes the transfer of sulphate from 3'-phosphoadenosine 5'-phosphosulphate to bile salts was purified from human liver cytosol by chromatography on DEAE-Sephadex and Sephadex G-200, by agarose suspension electrophoresis and by isoelectric focusing in free solution. The purified enzyme was also active towards oestrone, dehydroepiandrosterone and phenol. No other liver steroid sulphotransferases could be detected during this purification procedure. Km values of 1.8 . 10(-6) M and 3.3 . 10(-6) M for glycolithocholate and 3'-phosphoadenosine 5'-phosphosulphate respectively were found. The sulphotransferase has an isoelectric point of 5.5. The enzyme was markedly activated by Mg2+, Mn2+ and Co2+ and inhibited by Cu2+, Fe2+ and Zn2+. Chenodeoxycholate and deoxycholate were sulphated at the 7-OH and 12-OH position, respectively. No bile salt disulphate formation was detected. A 30-fold increase in specific activity was obtained, although the purification based on ultraviolet light measurements was considerably higher.

UI	MeSH Term	Description	Entries
D007525	Isoelectric Focusing	Electrophoresis in which a pH gradient is established in a gel medium and proteins migrate until they reach the site (or focus) at which the pH is equal to their isoelectric point.	Electrofocusing,Focusing, Isoelectric
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D008099	Liver	A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.	Livers
D008297	Male		Males
D010724	Phosphoadenosine Phosphosulfate	3'-Phosphoadenosine-5'-phosphosulfate. Key intermediate in the formation by living cells of sulfate esters of phenols, alcohols, steroids, sulfated polysaccharides, and simple esters, such as choline sulfate. It is formed from sulfate ion and ATP in a two-step process. This compound also is an important step in the process of sulfur fixation in plants and microorganisms.	Adenosine-3'-phosphate-5'-Phosphosulfate,Adenosine 3' phosphate 5' Phosphosulfate,Phosphosulfate, Phosphoadenosine
D002413	Cations, Divalent	Positively charged atoms, radicals or groups of atoms with a valence of plus 2, which travel to the cathode or negative pole during electrolysis.	Divalent Cations
D002850	Chromatography, Gel	Chromatography on non-ionic gels without regard to the mechanism of solute discrimination.	Chromatography, Exclusion,Chromatography, Gel Permeation,Chromatography, Molecular Sieve,Gel Filtration,Gel Filtration Chromatography,Chromatography, Size Exclusion,Exclusion Chromatography,Gel Chromatography,Gel Permeation Chromatography,Molecular Sieve Chromatography,Chromatography, Gel Filtration,Exclusion Chromatography, Size,Filtration Chromatography, Gel,Filtration, Gel,Sieve Chromatography, Molecular,Size Exclusion Chromatography
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D001647	Bile Acids and Salts	Steroid acids and salts. The primary bile acids are derived from cholesterol in the liver and usually conjugated with glycine or taurine. The secondary bile acids are further modified by bacteria in the intestine. They play an important role in the digestion and absorption of fat. They have also been used pharmacologically, especially in the treatment of gallstones.	Bile Acid,Bile Salt,Bile Salts,Bile Acids,Acid, Bile,Acids, Bile,Salt, Bile,Salts, Bile