Biomaterial Database

Enzymatic sulfation of bile salts. Partial purification and characterization of an enzyme from rat liver that catalyzes the sulfation of bile salts. 1977

L J Chen, and R J Bolt, and W H Admirand

An enzyme system which catalyzes the transfer of sulfate group from 3'-phosphoadenosine-5'-phosphosulfate to bile salts has been identified and characterized from rat liver. The enzyme is present in the cytosol fraction of liver cells. The apparent Km value for 3'-phosphoadenosine-5'-phosphosulfate was 8 - 10(-6) M andhat for taurolithocholate was 5 - 10(-5) M. Sulfation occurred with conjugated as well as unconjugated bile salts, however, the rate of sulfation was higher with conjugated than unconjugated. The enzyme was present in rat liver and kidney, but not detectable in brain, lung, heart, spleen or intestinal mucosa. The activity is completely inhibited by p-chloromercuribenzoate indicating the enzyme requires a sulfhydryl group for activity. A molecular weight of 130 000 was estimated by gel-filtration technique and the enzyme shows an isoelectric point of 5.3.

UI	MeSH Term	Description	Entries
D007668	Kidney	Body organ that filters blood for the secretion of URINE and that regulates ion concentrations.	Kidneys
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D008099	Liver	A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.	Livers
D009206	Myocardium	The muscle tissue of the HEART. It is composed of striated, involuntary muscle cells (MYOCYTES, CARDIAC) connected to form the contractile pump to generate blood flow.	Muscle, Cardiac,Muscle, Heart,Cardiac Muscle,Myocardia,Cardiac Muscles,Heart Muscle,Heart Muscles,Muscles, Cardiac,Muscles, Heart
D009928	Organ Specificity	Characteristic restricted to a particular organ of the body, such as a cell type, metabolic response or expression of a particular protein or antigen.	Tissue Specificity,Organ Specificities,Specificities, Organ,Specificities, Tissue,Specificity, Organ,Specificity, Tissue,Tissue Specificities
D010724	Phosphoadenosine Phosphosulfate	3'-Phosphoadenosine-5'-phosphosulfate. Key intermediate in the formation by living cells of sulfate esters of phenols, alcohols, steroids, sulfated polysaccharides, and simple esters, such as choline sulfate. It is formed from sulfate ion and ATP in a two-step process. This compound also is an important step in the process of sulfur fixation in plants and microorganisms.	Adenosine-3'-phosphate-5'-Phosphosulfate,Adenosine 3' phosphate 5' Phosphosulfate,Phosphosulfate, Phosphoadenosine
D001921	Brain	The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of three major parts including PROSENCEPHALON (the forebrain); MESENCEPHALON (the midbrain); and RHOMBENCEPHALON (the hindbrain). The developed brain consists of CEREBRUM; CEREBELLUM; and other structures in the BRAIN STEM.	Encephalon
D002729	Chloromercuribenzoates	Chloride and mercury-containing derivatives of benzoic acid.
D003600	Cytosol	Intracellular fluid from the cytoplasm after removal of ORGANELLES and other insoluble cytoplasmic components.	Cytosols
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia