-transparent.png){kind=logo}
Several metabolites detected in the bile of rats given radioactive retinoic acid were separated by liquid/gel partition chromatography and purified by high-pressure liquid chromatography. One of these metabolites was found to be sensitive to beta-D-glucuronidase, yielding both 13-cis- and all-trans-retinoic acid. It had the characteristic ultraviolet absorption spectrum of retinoic acid esters. Trimethylsilyl ether and acetyl derivatives of the methylated metabolite were prepared and examined by mass spectrometry. The resulting mass spectra established the structure to be retinoyl beta-glucuronide. Retinoyl glucuronide was rapidly excreted into the bile: the excretion was complete by 12 hr after the administration of retinoic acid. At this time the metabolite represented 12% of bile radioactivity (10% of dose). These observations confirm the existence of retinoyl glucuronide but demonstrate that it represents only one of several retinoic acid metabolites in bile.
M H Zile, and
H K Schnoes, and
H F DeLuca
January 1986,
Archives of biochemistry and biophysics,
M H Zile, and
H K Schnoes, and
H F DeLuca
January 1994,
Life sciences,
M H Zile, and
H K Schnoes, and
H F DeLuca
September 1995,
Drug metabolism and disposition: the biological fate of chemicals,
M H Zile, and
H K Schnoes, and
H F DeLuca
April 1965,
Science (New York, N.Y.),
M H Zile, and
H K Schnoes, and
H F DeLuca
February 1992,
Biochemical pharmacology,
M H Zile, and
H K Schnoes, and
H F DeLuca
February 1981,
Annals of the New York Academy of Sciences,
M H Zile, and
H K Schnoes, and
H F DeLuca
January 1993,
Teratology,
M H Zile, and
H K Schnoes, and
H F DeLuca
January 1990,
Journal of ocular pharmacology,
M H Zile, and
H K Schnoes, and
H F DeLuca
January 2004,
The Journal of nutrition,
M H Zile, and
H K Schnoes, and
H F DeLuca
July 2003,
Journal of pharmaceutical and biomedical analysis,
