Biomaterial Database

Isolation of human T lymphocytes: comparison between nylon wool filtration and rosetting with neuraminidase (VCN) and 2-aminoethylisothiouronium bromide (AET)-treated sheep red blood cells (SRBC). 1980

F Indiveri, and J Huddlestone, and M A Pellegrino, and S Ferrone

Isolation of human T lymphocytes by rosetting with SRBC, centrifugation on Ficoll-Hypaque gradients and lysis of SRBC, which reportedly affects the functional activities of lymphocytes, was compared to isolation of T lymphocytes by filtration through a nylon wool column. The two methods were similar in yields and purity of T lymphocytes. No selective loss of T cell subpopulations was found in T lymphocytes isolated by filtration through a nylon wool column as judged by the percentage of T gamma and T micron and the suppressor and helper activity in functional assays. Therefore, filtration through a nylon wool column is a useful method to purify T lymphocytes and has the advantage of being comparatively simple and quick to perform and of avoiding manipulations which may affect the functional activity of T lymphocytes.

UI	MeSH Term	Description	Entries
D007074	Immunoglobulin G	The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B.	Gamma Globulin, 7S,IgG,IgG Antibody,Allerglobuline,IgG(T),IgG1,IgG2,IgG2A,IgG2B,IgG3,IgG4,Immunoglobulin GT,Polyglobin,7S Gamma Globulin,Antibody, IgG,GT, Immunoglobulin
D008213	Lymphocyte Activation	Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION.	Blast Transformation,Blastogenesis,Lymphoblast Transformation,Lymphocyte Stimulation,Lymphocyte Transformation,Transformation, Blast,Transformation, Lymphoblast,Transformation, Lymphocyte,Activation, Lymphocyte,Stimulation, Lymphocyte
D009439	Neuraminidase	An enzyme that catalyzes the hydrolysis of alpha-2,3, alpha-2,6-, and alpha-2,8-glycosidic linkages (at a decreasing rate, respectively) of terminal sialic residues in oligosaccharides, glycoproteins, glycolipids, colominic acid, and synthetic substrate. (From Enzyme Nomenclature, 1992)	Sialidase,Exo-alpha-Sialidase,N-Acylneuraminate Glycohydrolases,Oligosaccharide Sialidase,Exo alpha Sialidase,Glycohydrolases, N-Acylneuraminate,N Acylneuraminate Glycohydrolases,Sialidase, Oligosaccharide
D002469	Cell Separation	Techniques for separating distinct populations of cells.	Cell Isolation,Cell Segregation,Isolation, Cell,Cell Isolations,Cell Segregations,Cell Separations,Isolations, Cell,Segregation, Cell,Segregations, Cell,Separation, Cell,Separations, Cell
D004912	Erythrocytes	Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN.	Blood Cells, Red,Blood Corpuscles, Red,Red Blood Cells,Red Blood Corpuscles,Blood Cell, Red,Blood Corpuscle, Red,Erythrocyte,Red Blood Cell,Red Blood Corpuscle
D005374	Filtration	A process of separating particulate matter from a fluid, such as air or a liquid, by passing the fluid carrier through a medium that will not pass the particulates. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)	Filtrations
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000338	beta-Aminoethyl Isothiourea	A radiation-protective agent that can inhibit DNA damage by binding to the DNA. It also increases the susceptibility of blood cells to complement-mediated lysis.	2-Aminoethylisothiuronium Bromide,beta-Aminoethylisothiuronium Diacetate,beta-Aminoethylisothiuronium Dihydrobromide,2-Aminoethylisothiuronium Diacetate,2-Aminoethylisothiuronium Dihydrochloride,2-Aminoethylisothiuronium Dihydroiodide,2-Aminoethylisothiuronium Diperchlorate,2-Aminoethylisothiuronium Monohydrobromide,2-Aminoethylisothiuronium Phosphate (1:1),2-Aminoethylisothiuronium Sulfate (1:1),AET,Aminoethylisothiuronium Bromide Hydrobromide,Carbamimidothioic acid, 2-aminoethyl ester, dihydrobromide,Isothiourea, (2-Aminoethyl),Ixecur,S-(2-Aminoethyl)isothiourea,beta-Aminoethylisothiuronium Bromide,beta-Aminoethylisothiuronium Dihydrochloride,beta-Aminoethylisothiuronium Dihydroiodide,beta-Aminoethylisothiuronium Diperchlorate,beta-Aminoethylisothiuronium Monohydrobromide,beta-Aminoethylisothiuronium Phosphate (1:1),beta-Aminoethylisothiuronium Sulfate (1:1),2 Aminoethylisothiuronium Bromide,2 Aminoethylisothiuronium Diacetate,2 Aminoethylisothiuronium Dihydrochloride,2 Aminoethylisothiuronium Dihydroiodide,2 Aminoethylisothiuronium Diperchlorate,2 Aminoethylisothiuronium Monohydrobromide,Bromide, 2-Aminoethylisothiuronium,Bromide, beta-Aminoethylisothiuronium,Diacetate, 2-Aminoethylisothiuronium,Diacetate, beta-Aminoethylisothiuronium,Dihydrobromide, beta-Aminoethylisothiuronium,Dihydrochloride, 2-Aminoethylisothiuronium,Dihydrochloride, beta-Aminoethylisothiuronium,Dihydroiodide, 2-Aminoethylisothiuronium,Dihydroiodide, beta-Aminoethylisothiuronium,Diperchlorate, 2-Aminoethylisothiuronium,Diperchlorate, beta-Aminoethylisothiuronium,Hydrobromide, Aminoethylisothiuronium Bromide,Isothiourea, beta-Aminoethyl,Monohydrobromide, 2-Aminoethylisothiuronium,Monohydrobromide, beta-Aminoethylisothiuronium,beta Aminoethyl Isothiourea,beta Aminoethylisothiuronium Bromide,beta Aminoethylisothiuronium Diacetate,beta Aminoethylisothiuronium Dihydrobromide,beta Aminoethylisothiuronium Dihydrochloride,beta Aminoethylisothiuronium Dihydroiodide,beta Aminoethylisothiuronium Diperchlorate,beta Aminoethylisothiuronium Monohydrobromide
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D012397	Rosette Formation	The in vitro formation of clusters consisting of a cell (usually a lymphocyte) surrounded by antigenic cells or antigen-bearing particles (usually erythrocytes, which may or may not be coated with antibody or antibody and complement). The rosette-forming cell may be an antibody-forming cell, a memory cell, a T-cell, a cell bearing surface cytophilic antibodies, or a monocyte possessing Fc receptors. Rosette formation can be used to identify specific populations of these cells.	Immunocytoadherence,Formation, Rosette,Formations, Rosette,Immunocytoadherences,Rosette Formations