Biomaterial Database

Pregnancy specific beta 1-glycoprotein (SP1) conversion of SP1 beta to SP1 alpha by "acidification": an artefact. 1982

B Teisner, and J G Grudzinskas

Late pregnancy serum and plasma were examined in rocket immunoelectrophoresis (RIE) and crossed immunoelectrophoresis (XIE) before and after "acid treatment" (pH 2.5, 10 min). The analysis revealed changes in the precipitation morphology in the majority of circulating proteins extending from the albumin to the gamma-region. In RIE, the precipitation pattern of SP1 was altered, this change being independent of polyethylene glycol. In addition, XIE analysis of SP1 revealed a change in the precipitation morphology with no change in electrophoretic mobility. The results can be explained by non-specific aggregation and denaturation due to "acid treatment", and are therefore unlikely to be of any biological or immunochemical importance.

UI	MeSH Term	Description	Entries
D007122	Immunoelectrophoresis	A technique that combines protein electrophoresis and double immunodiffusion. In this procedure proteins are first separated by gel electrophoresis (usually agarose), then made visible by immunodiffusion of specific antibodies. A distinct elliptical precipitin arc results for each protein detectable by the antisera.
D011247	Pregnancy	The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH.	Gestation,Pregnancies
D011257	Pregnancy Proteins	Proteins produced by organs of the mother or the PLACENTA during PREGNANCY. These proteins may be pregnancy-specific (present only during pregnancy) or pregnancy-associated (present during pregnancy or under other conditions such as hormone therapy or certain malignancies.)	Placental Proteins,Proteins, Placental,Proteins, Pregnancy
D011268	Pregnancy-Specific beta 1-Glycoproteins	Glycoproteins with the electrophoretic mobility of BETA-GLOBULINS, secreted by the placental TROPHOBLASTS into the maternal bloodstream during PREGNANCY. They can be detected 18 days after OVULATION and reach 200 mg/ml at the end of pregnancy. They are associated with fetal well-being.	Pregnancy-Specific beta 1-Glycoprotein,SP1 Pregnancy Protein,Trophoblast-Specific beta 1-Glycoprotein,beta 1-Glycoprotein, Pregnancy-Specific,beta-1 Glycoprotein, Trophoblast-Specific,PAPP-B,PAPP-C,PSBG-1,Pregnancy Specific beta 1 Glycoprotein,Pregnancy Specific beta-1 Glycoprotein,Pregnancy Specific beta1-Glycoprotein.,Pregnancy-Associated Plasma Protein B,Pregnancy-Associated Plasma Protein C,Pregnancy-Associated beta-Plasma Protein,Pregnancy-Specific beta-1 Globulin,Schwangerschaftsprotein 1,Trophoblastic beta 1-Glycoprotein,Trophoblastic beta(1)-Globulin,Trophoblastic beta-Globulin,beta 1-Globulin, Trophoblast-Specific,1-Glycoproteins, Pregnancy-Specific beta,PAPP B,PSBG 1,Pregnancy Associated Plasma Protein B,Pregnancy Associated Plasma Protein C,Pregnancy Protein, SP1,Pregnancy Specific beta 1 Globulin,Pregnancy Specific beta 1 Glycoproteins,Protein, SP1 Pregnancy,Trophoblast Specific beta 1 Glycoprotein,Trophoblast-Specific beta 1-Globulin,Trophoblast-Specific beta-1 Glycoprotein,Trophoblastic beta Globulin,beta 1 Globulin, Trophoblast Specific,beta 1-Glycoproteins, Pregnancy-Specific,beta-1 Globulin, Pregnancy-Specific,beta-Globulin, Trophoblastic
D011489	Protein Denaturation	Disruption of the non-covalent bonds and/or disulfide bonds responsible for maintaining the three-dimensional shape and activity of the native protein.	Denaturation, Protein,Denaturations, Protein,Protein Denaturations
D005260	Female		Females
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000143	Acids	Chemical compounds which yield hydrogen ions or protons when dissolved in water, whose hydrogen can be replaced by metals or basic radicals, or which react with bases to form salts and water (neutralization). An extension of the term includes substances dissolved in media other than water. (Grant & Hackh's Chemical Dictionary, 5th ed)	Acid