Biomaterial Database

Molecular heterogeneity of pregnancy-specific beta 1-glycoprotein (SP1). 1982

R M Hillier, and W R Jones

Ion-exchange chromatography has been used to isolate three separate forms of Pregnancy-Specific beta 1-Glycoprotein (SP1) from pooled, late-pregnancy human sera. Each of the three components was immunochemically identifiable as SP1, yet distinguishable from each other on the basis of rocket morphology resulting from rocket immunoelectrophoresis (RIE). The elution profile from DEAE Sephacel indicated that each component has an isoelectric point below pH 8.6, but suggested a different charge for each molecular species. Crossed immunoelectrophoresis (XIE) of pooled, late pregnancy serum suggested the presence of each component prior to physico-chemical treatment and showed the components to be characterised by alpha, beta or gamma electrophoretic mobility. The differences in rocket morphology suggested differing affinities for the antiserum, and a difference in molecular size, which was confirmed by gel filtration.

UI	MeSH Term	Description	Entries
D007123	Immunoelectrophoresis, Two-Dimensional	Immunoelectrophoresis in which a second electrophoretic transport is performed on the initially separated antigen fragments into an antibody-containing medium in a direction perpendicular to the first electrophoresis.	Immunoelectrophoresis, Crossed,Immunoelectrophoresis, 2-D,Immunoelectrophoresis, 2D,2-D Immunoelectrophoresis,2D Immunoelectrophoresis,Crossed Immunoelectrophoresis,Immunoelectrophoresis, 2 D,Immunoelectrophoresis, Two Dimensional,Two-Dimensional Immunoelectrophoresis
D008970	Molecular Weight	The sum of the weight of all the atoms in a molecule.	Molecular Weights,Weight, Molecular,Weights, Molecular
D011092	Polyethylene Glycols	Polymers of ETHYLENE OXIDE and water, and their ethers. They vary in consistency from liquid to solid depending on the molecular weight indicated by a number following the name. They are used as SURFACTANTS, dispersing agents, solvents, ointment and suppository bases, vehicles, and tablet excipients. Some specific groups are NONOXYNOLS, OCTOXYNOLS, and POLOXAMERS.	Macrogols,Polyoxyethylenes,Carbowax,Macrogol,Polyethylene Glycol,Polyethylene Oxide,Polyethyleneoxide,Polyglycol,Glycol, Polyethylene,Glycols, Polyethylene,Oxide, Polyethylene,Oxides, Polyethylene,Polyethylene Oxides,Polyethyleneoxides,Polyglycols,Polyoxyethylene
D011232	Chemical Precipitation	The formation of a solid in a solution as a result of a chemical reaction or the aggregation of soluble substances into complexes large enough to fall out of solution.	Precipitation, Chemical
D011247	Pregnancy	The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH.	Gestation,Pregnancies
D011257	Pregnancy Proteins	Proteins produced by organs of the mother or the PLACENTA during PREGNANCY. These proteins may be pregnancy-specific (present only during pregnancy) or pregnancy-associated (present during pregnancy or under other conditions such as hormone therapy or certain malignancies.)	Placental Proteins,Proteins, Placental,Proteins, Pregnancy
D011268	Pregnancy-Specific beta 1-Glycoproteins	Glycoproteins with the electrophoretic mobility of BETA-GLOBULINS, secreted by the placental TROPHOBLASTS into the maternal bloodstream during PREGNANCY. They can be detected 18 days after OVULATION and reach 200 mg/ml at the end of pregnancy. They are associated with fetal well-being.	Pregnancy-Specific beta 1-Glycoprotein,SP1 Pregnancy Protein,Trophoblast-Specific beta 1-Glycoprotein,beta 1-Glycoprotein, Pregnancy-Specific,beta-1 Glycoprotein, Trophoblast-Specific,PAPP-B,PAPP-C,PSBG-1,Pregnancy Specific beta 1 Glycoprotein,Pregnancy Specific beta-1 Glycoprotein,Pregnancy Specific beta1-Glycoprotein.,Pregnancy-Associated Plasma Protein B,Pregnancy-Associated Plasma Protein C,Pregnancy-Associated beta-Plasma Protein,Pregnancy-Specific beta-1 Globulin,Schwangerschaftsprotein 1,Trophoblastic beta 1-Glycoprotein,Trophoblastic beta(1)-Globulin,Trophoblastic beta-Globulin,beta 1-Globulin, Trophoblast-Specific,1-Glycoproteins, Pregnancy-Specific beta,PAPP B,PSBG 1,Pregnancy Associated Plasma Protein B,Pregnancy Associated Plasma Protein C,Pregnancy Protein, SP1,Pregnancy Specific beta 1 Globulin,Pregnancy Specific beta 1 Glycoproteins,Protein, SP1 Pregnancy,Trophoblast Specific beta 1 Glycoprotein,Trophoblast-Specific beta 1-Globulin,Trophoblast-Specific beta-1 Glycoprotein,Trophoblastic beta Globulin,beta 1 Globulin, Trophoblast Specific,beta 1-Glycoproteins, Pregnancy-Specific,beta-1 Globulin, Pregnancy-Specific,beta-Globulin, Trophoblastic
D002850	Chromatography, Gel	Chromatography on non-ionic gels without regard to the mechanism of solute discrimination.	Chromatography, Exclusion,Chromatography, Gel Permeation,Chromatography, Molecular Sieve,Gel Filtration,Gel Filtration Chromatography,Chromatography, Size Exclusion,Exclusion Chromatography,Gel Chromatography,Gel Permeation Chromatography,Molecular Sieve Chromatography,Chromatography, Gel Filtration,Exclusion Chromatography, Size,Filtration Chromatography, Gel,Filtration, Gel,Sieve Chromatography, Molecular,Size Exclusion Chromatography
D002852	Chromatography, Ion Exchange	Separation technique in which the stationary phase consists of ion exchange resins. The resins contain loosely held small ions that easily exchange places with other small ions of like charge present in solutions washed over the resins.	Chromatography, Ion-Exchange,Ion-Exchange Chromatography,Chromatographies, Ion Exchange,Chromatographies, Ion-Exchange,Ion Exchange Chromatographies,Ion Exchange Chromatography,Ion-Exchange Chromatographies
D005260	Female		Females