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Human epidermal growth factor (hEGF), which stimulates the growth of a variety of cells in culture, has recently been isolated from human urine. In the present study, we identified significant amounts of immunoreactive (IR) hEGF (mean +/- SE, 2.3 +/- 0.09 ng/ml; n = 3) in human pancreatic juice. The IR-hEGF materials were immunologically indistinguishable from standard hEGF, although they were only 5% as active in receptor binding to human placental membrane as in RIA. Gel exclusion chromatography of the pancreatic juice under neutral and acidic conditions revealed three distinct IR-hEGF components with different molecular sizes. Incubation of 125I-labeled hEGF with either the pancreatic juice or the high molecular weight component(s) yielded no aggregation or degradation products. These data suggest that fully immunoreactive but much less bioactive hEGF-like substances which are heterogeneous in size are secreted into human pancreatic juice. Their tissue(s) of origin and physiological functions, if any, remain to be elucidated.
Y Hirata, and
M Uchihashi, and
M Nakajima, and
T Fujita, and
S Matsukura
June 1989,
The Journal of clinical endocrinology and metabolism,
Y Hirata, and
M Uchihashi, and
M Nakajima, and
T Fujita, and
S Matsukura
January 1978,
The Mount Sinai journal of medicine, New York,
Y Hirata, and
M Uchihashi, and
M Nakajima, and
T Fujita, and
S Matsukura
April 1990,
The American journal of gastroenterology,
Y Hirata, and
M Uchihashi, and
M Nakajima, and
T Fujita, and
S Matsukura
March 1980,
The Journal of clinical endocrinology and metabolism,
Y Hirata, and
M Uchihashi, and
M Nakajima, and
T Fujita, and
S Matsukura
January 1988,
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society,
Y Hirata, and
M Uchihashi, and
M Nakajima, and
T Fujita, and
S Matsukura
April 1991,
Clinical biochemistry,
Y Hirata, and
M Uchihashi, and
M Nakajima, and
T Fujita, and
S Matsukura
April 1996,
Gut,
Y Hirata, and
M Uchihashi, and
M Nakajima, and
T Fujita, and
S Matsukura
February 1991,
The Journal of pathology,
Y Hirata, and
M Uchihashi, and
M Nakajima, and
T Fujita, and
S Matsukura
January 1992,
The Journal of pathology,
Y Hirata, and
M Uchihashi, and
M Nakajima, and
T Fujita, and
S Matsukura
November 1996,
Digestive diseases and sciences,
