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[14C]aflatoxin B1 (AFB1) was injected i.p. into female Wistar rats. Half of the dose was eliminated into the bile mostly as polar non-extractable metabolites. Among these a glutathione conjugate was the main component. The same conjugate was formed when rat liver postmitochondrial supernatant was incubated with AFB1 and [3H]glutathione. The conjugate was purified by ion exchange chromatography, gel-filtration and thin layer chromatography (TLC). It was tentatively identified as 2,3-dihydro-2-(S-glutathionyl)-3-hydroxy aflatoxin B1 (AFB1-GSH-conjugate). This structure was derived mainly from amino acid analysis, ultraviolet spectra and the enzymatic requirements for its formation in in vitro experiments. In the rat this detoxification product of the potentially ultimate reactive AFB1-epoxide constitutes about 10% of the administered dose and thus underlines the quantitative importance of this activating pathway.
G H Degen, and
H G Neumann
April 1978,
Science (New York, N.Y.),
G H Degen, and
H G Neumann
January 1981,
European journal of drug metabolism and pharmacokinetics,
G H Degen, and
H G Neumann
October 1985,
Chemico-biological interactions,
G H Degen, and
H G Neumann
August 1974,
Research communications in chemical pathology and pharmacology,
G H Degen, and
H G Neumann
January 1974,
Journal of agricultural and food chemistry,
G H Degen, and
H G Neumann
December 1979,
Xenobiotica; the fate of foreign compounds in biological systems,
G H Degen, and
H G Neumann
January 1991,
Chemico-biological interactions,
G H Degen, and
H G Neumann
October 1975,
Chemico-biological interactions,
G H Degen, and
H G Neumann
January 1983,
The Biochemical journal,
G H Degen, and
H G Neumann
April 1971,
Chemical & pharmaceutical bulletin,
