The biotension generated by cells and tissues is connected quite well with the intensity of the metabolic processes in them and in seems that it is affected considerably by a series of external factors-temperature, salt content ect. For the purposes of transplantation in the present investigation the authors examined the effect of the nutritive medium for tissue cultures T-199 on the investigated biotension of the corneal tissue during the period of its preservation. They carried out studies on 335 corneas and the results were estimated statistically. The mean rate of a change on the biotension for the single series of investigation was determined. He found that the measured biotension was affected bu the time and conditions of preservation of the corneal tissue, when he compared the dispersion in the values of the tension after the preservation of the tissue in two media moist chamber and nutritive medium for tissue cultures). The nutritive medium used probably influenced positively the rate of the adaptation processes in the tissues after its separation from the ocular buebus, delayed the processes of deterioration and to a large extent stabilized the measured biotension.