Biomaterial Database

An improved embedding method for electron microscopy of lepromata. 1980

S Okada, and Y Fukunishi, and A Mukherjee, and G Ramu, and K V Desikan

Spurr's resin mixture has been found to be the embedding material most suitable for electron microscopic studies of lepromata at present. Like methacrylate, it can penetrate well into foamy structures and the leprosy bacilli within foamy structures. On the other hand, like Epon 812, it is stable against the electron beam and can preserve ultrafine structures. Additionally, we have found that the use of dimethylformamide or dimethylsulfoxide instead of propylene oxide as substituter improves the preservation of the ultrafine structures of leprosy bacilli.

UI	MeSH Term	Description	Entries
D007918	Leprosy	A chronic granulomatous infection caused by MYCOBACTERIUM LEPRAE. The granulomatous lesions are manifested in the skin, the mucous membranes, and the peripheral nerves. Two polar or principal types are lepromatous and tuberculoid.	Hansen's Disease,Hansen Disease
D008689	Methacrylates	Acrylic acids or acrylates which are substituted in the C-2 position with a methyl group.	Methacrylate
D008854	Microscopy, Electron	Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.	Electron Microscopy
D009166	Mycobacterium leprae	A species of gram-positive, aerobic bacteria that causes LEPROSY in man. Its organisms are generally arranged in clumps, rounded masses, or in groups of bacilli side by side.
D012116	Resins, Plant	Flammable, amorphous, vegetable products of secretion or disintegration, usually formed in special cavities of plants. They are generally insoluble in water and soluble in alcohol, carbon tetrachloride, ether, or volatile oils. They are fusible and have a conchoidal fracture. They are the oxidation or polymerization products of the terpenes, and are mixtures of aromatic acids and esters. Most are soft and sticky, but harden after exposure to cold. (From Grant & Hackh's Chemical Dictionary, 5th ed & Dorland, 28th ed)	Plant Resins
D004853	Epoxy Resins	Polymeric resins derived from OXIRANES and characterized by strength and thermosetting properties. Epoxy resins are often used as dental materials.	Epoxy Resin,Resin, Epoxy,Resins, Epoxy
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000042	Absorption	The physical or physiological processes by which substances, tissue, cells, etc. take up or take in other substances or energy.