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Isotope dilution/mass spectrometric methods for total serum cholesterol, developed separately at the Karolinska Institutet (KI) and the National Bureau of Standards (NBS), were compared by applying them to a common set of serum pools. A search for the cause of a systematic difference of a few percent in results from the two methods revealed that the KI cholesterol standard contained lathosterol, which interfered with the calibration of the method. With NBS Standard Reference Material cholesterol used for new analyses at the KI, the average difference in mean values dropped to 0.2%. The NBS results are more precise. This is attributed to the protocols NBS used for sample preparation and mass spectrometry. However, these protocols make the NBS method more complex and time-consuming. A recent critical article on the use of this technique for total cholesterol is also examined.
R Schaffer, and
L T Sniegoski, and
M J Welch, and
V E White, and
A Cohen, and
H S Hertz, and
J Mandel, and
R C Paule, and
L Svensson, and
I Björkhem, and
R Blomstrand
August 1989,
Analytical chemistry,
R Schaffer, and
L T Sniegoski, and
M J Welch, and
V E White, and
A Cohen, and
H S Hertz, and
J Mandel, and
R C Paule, and
L Svensson, and
I Björkhem, and
R Blomstrand
September 2003,
Analytical chemistry,
R Schaffer, and
L T Sniegoski, and
M J Welch, and
V E White, and
A Cohen, and
H S Hertz, and
J Mandel, and
R C Paule, and
L Svensson, and
I Björkhem, and
R Blomstrand
October 1997,
Clinical chemistry,
R Schaffer, and
L T Sniegoski, and
M J Welch, and
V E White, and
A Cohen, and
H S Hertz, and
J Mandel, and
R C Paule, and
L Svensson, and
I Björkhem, and
R Blomstrand
March 1964,
The Journal of laboratory and clinical medicine,
R Schaffer, and
L T Sniegoski, and
M J Welch, and
V E White, and
A Cohen, and
H S Hertz, and
J Mandel, and
R C Paule, and
L Svensson, and
I Björkhem, and
R Blomstrand
January 1981,
Biomedical mass spectrometry,
R Schaffer, and
L T Sniegoski, and
M J Welch, and
V E White, and
A Cohen, and
H S Hertz, and
J Mandel, and
R C Paule, and
L Svensson, and
I Björkhem, and
R Blomstrand
October 1988,
Analytical chemistry,
R Schaffer, and
L T Sniegoski, and
M J Welch, and
V E White, and
A Cohen, and
H S Hertz, and
J Mandel, and
R C Paule, and
L Svensson, and
I Björkhem, and
R Blomstrand
June 1988,
The Analyst,
R Schaffer, and
L T Sniegoski, and
M J Welch, and
V E White, and
A Cohen, and
H S Hertz, and
J Mandel, and
R C Paule, and
L Svensson, and
I Björkhem, and
R Blomstrand
January 2002,
Rapid communications in mass spectrometry : RCM,
R Schaffer, and
L T Sniegoski, and
M J Welch, and
V E White, and
A Cohen, and
H S Hertz, and
J Mandel, and
R C Paule, and
L Svensson, and
I Björkhem, and
R Blomstrand
January 1980,
Methods in enzymology,
R Schaffer, and
L T Sniegoski, and
M J Welch, and
V E White, and
A Cohen, and
H S Hertz, and
J Mandel, and
R C Paule, and
L Svensson, and
I Björkhem, and
R Blomstrand
January 1974,
Vutreshni bolesti,
