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Human skin was labeled with purified antibodies against type II and III collagens and against their extension aminopropeptides by using the ferritin technique. Both aminopropeptides were visualized mainly along thin collagenous fibrils (diameter, 20-40 nm) and rarely in nonfibrillar regions of the skin. The labeling showed a periodicity of 60-65 nm resembling the D (67 nm) stagger of collagen molecules. Blocking of antibodies with aminopropeptides and treatment of tissues with procollagen NH2-terminal protease abolished labeling. Antibodies against type I collagen uniformly labeled approximately equal to 80% of the fibrils (diameter, 20-80 nm), while reaction with antibodies against type III collagen was restricted to thin fibrils. It is currently thought that the aminopropeptides of procollagen molecules are cleaved after they are released from the cell and before fibril formation. Our data indicate that aminopropeptides are removed at the fibrillar level and that fibril growth can be regulated by extracellular procollagen processing.
R Fleischmajer, and
R Timpl, and
L Tuderman, and
L Raisher, and
M Wiestner, and
J S Perlish, and
P N Graves
December 1976,
The Biochemical journal,
R Fleischmajer, and
R Timpl, and
L Tuderman, and
L Raisher, and
M Wiestner, and
J S Perlish, and
P N Graves
July 2000,
Scandinavian journal of gastroenterology,
R Fleischmajer, and
R Timpl, and
L Tuderman, and
L Raisher, and
M Wiestner, and
J S Perlish, and
P N Graves
October 1987,
Pathologie-biologie,
R Fleischmajer, and
R Timpl, and
L Tuderman, and
L Raisher, and
M Wiestner, and
J S Perlish, and
P N Graves
January 1988,
Acta dermato-venereologica,
R Fleischmajer, and
R Timpl, and
L Tuderman, and
L Raisher, and
M Wiestner, and
J S Perlish, and
P N Graves
February 1998,
The American Journal of dermatopathology,
R Fleischmajer, and
R Timpl, and
L Tuderman, and
L Raisher, and
M Wiestner, and
J S Perlish, and
P N Graves
February 1976,
Proceedings of the National Academy of Sciences of the United States of America,
R Fleischmajer, and
R Timpl, and
L Tuderman, and
L Raisher, and
M Wiestner, and
J S Perlish, and
P N Graves
June 1977,
Biochimica et biophysica acta,
R Fleischmajer, and
R Timpl, and
L Tuderman, and
L Raisher, and
M Wiestner, and
J S Perlish, and
P N Graves
April 1980,
Journal of chromatography,
R Fleischmajer, and
R Timpl, and
L Tuderman, and
L Raisher, and
M Wiestner, and
J S Perlish, and
P N Graves
November 1987,
The Biochemical journal,
R Fleischmajer, and
R Timpl, and
L Tuderman, and
L Raisher, and
M Wiestner, and
J S Perlish, and
P N Graves
November 1990,
Experimental cell research,
