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Two substrains of Escherichia coli B/r were grown to steady-state in batch cultures at temperatures between 22 and 42 degrees C in different growth media. The size and shape of the cells were measured from light and electron micrographs and with the Coulter channelizer. The results indicate that cells are shorter and somewhat thicker at the lower temperatures, especially in rich growth media; cell volume is then slightly smaller. A lower temperature was further found to increase the relative duration of the constriction period. The shapes of the cell size distributions are indistinguishable, indicating that the pattern of growth of the cells is the same at all temperatures. The adaptation of the cells to a temperature shift lasted several generations, indicating that the morphological effects of temperature are mediated by the cell's physiology.
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F J Trueba, and
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F J Trueba, and
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F J Trueba, and
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F J Trueba, and
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