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Improved methods are described for the fluorimetric determination of 5-hydroxytryptamine (5-HT), 5-hydroxyindoleacetic acid (5-HIAA), and tryptophan (TRP) in plasma, platelet and RBC blood compartments. The methods for 5-HT and 5-HIAA utilize ion exchange chromatography and allow simultaneous determinations to be made on the same blood sample. The tryptophan assay is a modification of an existing extraction method. All of the 5-HT in whole blood was found to be associated with the platelet fraction (122.6 ng/ml), whereas all 5-HIAA was distributed between the plasma (78.3 ng/ml) and RBC (124.0 ng/ml) compartments. Tryptophan was found in all three compartments although the majority of this amino acid was in the plasma (4.3 microgram/ml) and RBC (1.7 microgram/ml) fractions.
E M Demet, and
A E Halaris, and
S Bhatarakamol
March 1997,
Current opinion in hematology,
E M Demet, and
A E Halaris, and
S Bhatarakamol
December 1982,
Biochimica et biophysica acta,
E M Demet, and
A E Halaris, and
S Bhatarakamol
November 1986,
Neurochemical research,
E M Demet, and
A E Halaris, and
S Bhatarakamol
January 1995,
Life sciences,
E M Demet, and
A E Halaris, and
S Bhatarakamol
February 2014,
The journal of physical chemistry letters,
E M Demet, and
A E Halaris, and
S Bhatarakamol
March 2011,
IUBMB life,
E M Demet, and
A E Halaris, and
S Bhatarakamol
April 1972,
Biochemical pharmacology,
E M Demet, and
A E Halaris, and
S Bhatarakamol
October 1987,
Journal of immunology (Baltimore, Md. : 1950),
E M Demet, and
A E Halaris, and
S Bhatarakamol
November 1987,
Cancer research,
E M Demet, and
A E Halaris, and
S Bhatarakamol
July 1981,
Neurochemical research,
