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Nuclei from concanavalin-A-activated lymphocytes were used to study the replication of chromatin in vitro. Micrococcal nuclease was employed to obtain information about the structure of the replicated chromatin. The nuclease digestion products were examined by sucrose gradient sedimentation and by gel electrophoresis. Experiments are presented which indicate that DNA replicated in vitro is organized into chromatin whose structure is similar to that of bulk chromatin. This conclusion is based on the following observations: (a) DNA replicated in vitro is associated with typical chromatin subunits (nucleosomes) even after short replication times, when the newly replicated DNA consists almost entirely of Okazaki fragments; (b) the length of internucleosomal spacer DNA in part of the replicated chromatin corresponds to that in bulk chromatin. Evidence which suggests that the structure of nucleosomes is transiently altered in the vicinity of the replication fork is presented.
E J Schlaeger, and
K H Klempnauer
September 1970,
Biochemical and biophysical research communications,
E J Schlaeger, and
K H Klempnauer
January 1974,
Cold Spring Harbor symposia on quantitative biology,
E J Schlaeger, and
K H Klempnauer
March 1982,
Journal of molecular biology,
E J Schlaeger, and
K H Klempnauer
December 1991,
Molecular and cellular biology,
E J Schlaeger, and
K H Klempnauer
March 1997,
Journal of molecular biology,
E J Schlaeger, and
K H Klempnauer
January 1998,
Annual review of biophysics and biomolecular structure,
E J Schlaeger, and
K H Klempnauer
March 1984,
Biochemistry,
E J Schlaeger, and
K H Klempnauer
December 1982,
Biochimica et biophysica acta,
E J Schlaeger, and
K H Klempnauer
May 2004,
Methods (San Diego, Calif.),
