The ability of platelet concentrates stored at room temperature to withstand transportation after removal from the rotator and ideal temperature environment was studied by reinfusing autologous 51Cr-labeled platelets obtained from normal volunteers. Periods of up to 6 hours off the rotator and not under temperature control did not result in a significant change in platelet viability. Platelets transported up to 12 hours after 24 hours of storage and then placed on a rotator for the remainder of the 72-hour storage interval also showed expected recovery and survival. Thus, room temperature stored platelets may be used even when transportation for up to 6 hours is required before the concentrates are transfused.