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This study reports the presence of glycylprolyl dipeptidyl aminopeptidase in porcine pancreas, and its partial purification and some properties. Crude enzyme preparation was obtained by extraction from acetone-dried powder of the pancreas at pH 7.6. For solubilization of enzyme, freezing and thawing were carried out. Crude enzyme extract was fractionated with ammonium sulfate precipitation, gel filtration on Sephadex G-200 column and ion-exchange chromatography on DEAE-cellulose. Partially purified enzyme showed 2897-folds purification. The enzyme activity on polyacrylamide gel electrophoresis showed good agreement with a main protein band stained with Coomassie brilliant blue. Molecular weight of this enzyme from the pancreas was estimated to be 300000 by gel filtration on Sephacryl S-300 column. Optimum pH was between 8.5 and 9.0, and Km value for glycylproline-p-nitroanilide tosilate was 0.33 mM. This enzyme from the pancreas was a serine enzyme and was relatively stable to heat at 60 degrees C for 10 min.
M Ichinose, and
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B Watanabe, and
T Ishimaru, and
M Izumi, and
S Miyake, and
M Takamori
November 2004,
Nihon rinsho. Japanese journal of clinical medicine,
M Ichinose, and
R Maeda, and
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M Izumi, and
S Miyake, and
M Takamori
January 1998,
Nihon rinsho. Japanese journal of clinical medicine,
M Ichinose, and
R Maeda, and
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M Takamori
August 1999,
Nihon rinsho. Japanese journal of clinical medicine,
M Ichinose, and
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B Watanabe, and
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M Takamori
December 1989,
Oral microbiology and immunology,
M Ichinose, and
R Maeda, and
T Fukuda, and
B Watanabe, and
T Ishimaru, and
M Izumi, and
S Miyake, and
M Takamori
July 1982,
Journal of biochemistry,
M Ichinose, and
R Maeda, and
T Fukuda, and
B Watanabe, and
T Ishimaru, and
M Izumi, and
S Miyake, and
M Takamori
December 1986,
Biochemistry,
M Ichinose, and
R Maeda, and
T Fukuda, and
B Watanabe, and
T Ishimaru, and
M Izumi, and
S Miyake, and
M Takamori
February 1990,
Applied and environmental microbiology,
M Ichinose, and
R Maeda, and
T Fukuda, and
B Watanabe, and
T Ishimaru, and
M Izumi, and
S Miyake, and
M Takamori
November 1992,
Acta paediatrica (Oslo, Norway : 1992),
M Ichinose, and
R Maeda, and
T Fukuda, and
B Watanabe, and
T Ishimaru, and
M Izumi, and
S Miyake, and
M Takamori
October 1983,
Nihon Ika Daigaku zasshi,
M Ichinose, and
R Maeda, and
T Fukuda, and
B Watanabe, and
T Ishimaru, and
M Izumi, and
S Miyake, and
M Takamori
May 1986,
Journal of general microbiology,
