A 21-day subchronic dermal toxicity of trifluoropropylmethylcyclotrisiloxane (TFP) was conducted in New Zealand white rabbits. One control and three treatment groups of male and female animals were administered with the test material at levels of 0, 40, 200 and 400 mg/kg/day for three weeks. Mortality, behavioral reactions, growth and food consumption were observed and measured along with hematology, clinical blood chemistry, relative organ weight and pathology parameters. Five animals died at the highest dose level during the test period. Treatment with two higher dosages generally resulted in reduced rate of weight gain, lower food consumption, depressed serum alkaline phosphatase activity and increased activity of the serum enzymes. No significant adverse effects were observed at lower levels of 40 mg/kg. Gross pathologic examination revealed no treatment related causes of death and all organs and tissues were observed normal at the time of necropsy. There were no chemical related microscopic changes found in any of the test animals.