-transparent.png){kind=logo}
The formation of the 7, 8-a and 9, 10-dihydrodiol metabolites of benzo(a)pyrene, which are believed to play a role in the chemical induction of tumors, was investigated in cultures of human and murine origin. It was found that cultures of mouse (strain C3Hz) epidermal and skin fibroblastic cells showed inducible benzo(a)pyrene metabolism towards dihydrodiol metabolites, after pre-incubation with benz(a)anthracene. This was consistent with the increased in vivo formation of dihydrodiol metabolites of benzo(a)pyrene after injection with 3-methylcholanthrene. In contrast, in human cell cultures the metabolism of benzo(a)pyrene to the dihydrodiol metabolites was not enhanced after pre-exposure to benz(a)anthracene. This was the case in low-passage skin fibroblasts, primary epidermal skin cells, and primary keratinocytes from hair follicles. Moreover, other inducers of microsomal oxygenases, such as phenobarbital and 3-methylcholanthrene, were also unable to increase benzo(a)pyrene metabolism towards the dihydrodiol compounds. In view of these results, obtained using in vitro human and murine model systems, we may conclude that human and murine skin cell culture systems respond differently to pre-treatment with inducers of microsomal monooxygenases with respect to the metabolism of benzo(a)pyrene to reactive dihydrodiol metabolites. The possible implications for the human in vivo situation are discussed.
M W Hukkelhoven, and
E Vromans, and
A J Vermorken, and
H Bloemendal
June 1983,
Toxicology letters,
M W Hukkelhoven, and
E Vromans, and
A J Vermorken, and
H Bloemendal
August 1998,
Toxicology and applied pharmacology,
M W Hukkelhoven, and
E Vromans, and
A J Vermorken, and
H Bloemendal
March 1986,
Cell biology and toxicology,
M W Hukkelhoven, and
E Vromans, and
A J Vermorken, and
H Bloemendal
March 2001,
Carcinogenesis,
M W Hukkelhoven, and
E Vromans, and
A J Vermorken, and
H Bloemendal
September 1991,
Carcinogenesis,
M W Hukkelhoven, and
E Vromans, and
A J Vermorken, and
H Bloemendal
October 1979,
Cancer research,
M W Hukkelhoven, and
E Vromans, and
A J Vermorken, and
H Bloemendal
January 1982,
Carcinogenesis,
M W Hukkelhoven, and
E Vromans, and
A J Vermorken, and
H Bloemendal
April 1996,
Cancer letters,
M W Hukkelhoven, and
E Vromans, and
A J Vermorken, and
H Bloemendal
January 1983,
Chemico-biological interactions,
M W Hukkelhoven, and
E Vromans, and
A J Vermorken, and
H Bloemendal
January 1978,
Pharmacology,
