Biomaterial Database

Uptake of galactose, ouabain and taurocholate into centrilobular and periportal enriched hepatocyte subpopulations. 1981

N H Stacey, and C D Klaassen

Rat hepatocytes were isolated by a collagenase perfusion technique with subsequent subfractionation on Metrizamide gradients into subpopulations which have been designated band I and band II and are likely to be enriched with centrilobular and periportal cells, respectively. Band I was found to have a higher concentration of 5'-nucleotidase and band II a higher concentration of alcohol dehydrogenase. Furthermore, pretreatment of rats with phenobarbital led to higher cytochrome P-450 in the band I (centrilobular enriched) as compared to the band II (periportal enriched) subpopulations of hepatocytes. These data support their ascribed lobular origins. The uptake of a single concentration of galactose, ouabain and taurocholate into each of the two subpopulations was investigated until the concentration within the hepatocytes no longer increased. No difference was found in the uptake of [14C]galactose (25 mM) between the two hepatocyte subpopulations. However, the uptake of [3H]ouabain (125 microM) was greater in the centrilobular as compared to periportal enriched fraction of the hepatocytes. An even greater difference was found for the uptake of [3H]taurocholate (25 microM). The kinetics of taurocholate uptake were subsequently investigated. The Km for each subpopulation was 21 microM, while the Vmax of the centrilobular enriched fraction was 2.03 and that of the periportal enriched fraction was 1.57 nmol/min/mg of protein. These results show that there is a difference in uptake into hepatocytes of centrilobular and periportal origin for ouabain and taurocholate, but not for galactose.

UI	MeSH Term	Description	Entries
D008099	Liver	A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.	Livers
D008297	Male		Males
D010042	Ouabain	A cardioactive glycoside consisting of rhamnose and ouabagenin, obtained from the seeds of Strophanthus gratus and other plants of the Apocynaceae; used like DIGITALIS. It is commonly used in cell biological studies as an inhibitor of the NA(+)-K(+)-EXCHANGING ATPASE.	Acocantherin,G-Strophanthin,Acolongifloroside K,G Strophanthin
D002458	Cell Fractionation	Techniques to partition various components of the cell into SUBCELLULAR FRACTIONS.	Cell Fractionations,Fractionation, Cell,Fractionations, Cell
D005690	Galactose	An aldohexose that occurs naturally in the D-form in lactose, cerebrosides, gangliosides, and mucoproteins. Deficiency of galactosyl-1-phosphate uridyltransferase (GALACTOSE-1-PHOSPHATE URIDYL-TRANSFERASE DEFICIENCY DISEASE) causes an error in galactose metabolism called GALACTOSEMIA, resulting in elevations of galactose in the blood.	D-Galactose,Galactopyranose,Galactopyranoside,D Galactose
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D013656	Taurocholic Acid	The product of conjugation of cholic acid with taurine. Its sodium salt is the chief ingredient of the bile of carnivorous animals. It acts as a detergent to solubilize fats for absorption and is itself absorbed. It is used as a cholagogue and cholerectic.	Cholyltaurine,Taurine Cholate,Taurocholate,Sodium Taurocholate,Taurocholate Sodium,Taurocholic Acid, (5 alpha)-Isomer,Taurocholic Acid, (7 beta)-Isomer,Taurocholic Acid, Monolithium Salt,Taurocholic Acid, Monosodium Salt,Taurocholate, Sodium
D051381	Rats	The common name for the genus Rattus.	Rattus,Rats, Laboratory,Rats, Norway,Rattus norvegicus,Laboratory Rat,Laboratory Rats,Norway Rat,Norway Rats,Rat,Rat, Laboratory,Rat, Norway,norvegicus, Rattus
D066298	In Vitro Techniques	Methods to study reactions or processes taking place in an artificial environment outside the living organism.	In Vitro Test,In Vitro Testing,In Vitro Tests,In Vitro as Topic,In Vitro,In Vitro Technique,In Vitro Testings,Technique, In Vitro,Techniques, In Vitro,Test, In Vitro,Testing, In Vitro,Testings, In Vitro,Tests, In Vitro,Vitro Testing, In