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A computerized quantitative technique was used to analyze the distribution of ferritininsulin receptor sites on rat adipocytes and the effects of cytochalasin B on groups of receptor sites. Computer analysis of separation distances between receptor sites established that insulin receptor sites on adipocytes did not have a random distribution but have a distinct tendency to exist in groups with a maximum separation distance between particles of 400 A. A peak in the distribution of separation distances occurred at 100-200 A. Cytochalasin B, but not cytochalasin D, treatment of adipocytes resulted in a decrease in the number of large groups of receptor sites and a corresponding increase in single and paired receptor sites without affecting the separation distance between the remaining grouped receptors. This suggested that when cytochalasin B disrupted the bond holding receptor sites together, it caused complete disruption. These observations provided additional information on the ultrastructural characteristics of the insulin receptor. Further application of these techniques to the analysis of insulin receptors may provide the necessary structural correlates to the biochemically observed differences in insulin action in other tissues and diseased states.
N D Gershon, and
R M Smith, and
L Jarett
April 1979,
The Journal of clinical investigation,
N D Gershon, and
R M Smith, and
L Jarett
October 1980,
European journal of cell biology,
N D Gershon, and
R M Smith, and
L Jarett
February 1988,
Molecular immunology,
N D Gershon, and
R M Smith, and
L Jarett
March 1986,
Diabetes,
N D Gershon, and
R M Smith, and
L Jarett
August 1982,
Acta medica Okayama,
N D Gershon, and
R M Smith, and
L Jarett
January 1980,
Scanning electron microscopy,
N D Gershon, and
R M Smith, and
L Jarett
November 1977,
The Journal of clinical investigation,
N D Gershon, and
R M Smith, and
L Jarett
January 1990,
Journal of receptor research,
N D Gershon, and
R M Smith, and
L Jarett
March 1979,
British journal of pharmacology,
N D Gershon, and
R M Smith, and
L Jarett
September 1978,
Diabetes,
