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Glucosoisomerase isolated from disrupted cells of Lactobacillus brevis 74 by extraction with K, Na phosphate buffer was purified by step-by-step fractionation with (NH4)2SO4 and DEAE-cellulose chromatography. The purified enzyme was examined by polyacrylamide gel electrophoresis and ultracentrifugation. The examination showed the enzyme to be homogeneous. The sedimentation constant of the enzyme was found to be 8.3 S. The molecular weight of the enzyme was estimated to be 200 000. The presence of the carbohydrate moiety in the enzyme was demonstrated by qualitative methods.
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