Formation of N-nitrosamine and N-nitrosamino acids from food products and nitrite under simulated gastric conditions. 1980

P J Groenen, and M W de Cock-Bethbeder, and J Bouwman, and J H Dhont

Average-sized portions of a variety of food products were reacted with nitrite under realistically simulated gastric conditions. The aqueous incubation medium contained sodium nitrite (10 mg/l) and potassium thiocyanate to mimic the incoming flux of saliva, as well as pepsin, sodium chloride and hydrochloric acid, reflecting the composition of gastric juice. After incubation for 2 hr at 37 degrees C, volatile N-nitrosamines and N-nitrosamino acids were determined in the reaction mixtures. Nitrosodimethylamine (NDMA) was present in the incubation mixtures of smoked mackerel (8.5 micrograms per portion), canned herring (0.66 micrograms per portion) and beer (0.70 micrograms per 'portion'). Smaller amounts per portion, sometimes of other nitrosamines as well, were observed with canned salmon and anchovy, mustard, yoghurt and coffee brew. Negative results were obtained for canned tuna, soya sauce, ketchup, white bread, 'nasi goreng', tea brew and cocoa milk. Nitrosamino acids were detected in the reaction mixtures of smoked mackerel (58 micrograms per portion), soya sauce (24 micrograms per portion) and canned salmon (6.9 micrograms per portion) and in smaller amounts in those of canned herring, anchovy and cocoa milk. In order to reduce the number of analyses to be performed, most products have been studied only after incubation, so that the nitrosamines and nitrosamino acids found may already have been present -- wholly or partly -- in the original products, before incubation. Such is the case for part of the NDMA in the reaction mixture of smoked mackerel and for all the NDMA in beer. The toxicological implications of these findings remain to be established.

UI MeSH Term Description Entries
D008401 Gas Chromatography-Mass Spectrometry A microanalytical technique combining mass spectrometry and gas chromatography for the qualitative as well as quantitative determinations of compounds. Chromatography, Gas-Liquid-Mass Spectrometry,Chromatography, Gas-Mass Spectrometry,GCMS,Spectrometry, Mass-Gas Chromatography,Spectrum Analysis, Mass-Gas Chromatography,Gas-Liquid Chromatography-Mass Spectrometry,Mass Spectrometry-Gas Chromatography,Chromatography, Gas Liquid Mass Spectrometry,Chromatography, Gas Mass Spectrometry,Chromatography, Mass Spectrometry-Gas,Chromatography-Mass Spectrometry, Gas,Chromatography-Mass Spectrometry, Gas-Liquid,Gas Chromatography Mass Spectrometry,Gas Liquid Chromatography Mass Spectrometry,Mass Spectrometry Gas Chromatography,Spectrometries, Mass-Gas Chromatography,Spectrometry, Gas Chromatography-Mass,Spectrometry, Gas-Liquid Chromatography-Mass,Spectrometry, Mass Gas Chromatography,Spectrometry-Gas Chromatography, Mass,Spectrum Analysis, Mass Gas Chromatography
D008954 Models, Biological Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment. Biological Model,Biological Models,Model, Biological,Models, Biologic,Biologic Model,Biologic Models,Model, Biologic
D009573 Nitrites Salts of nitrous acid or compounds containing the group NO2-. The inorganic nitrites of the type MNO2 (where M Nitrite
D009602 Nitrosamines A class of compounds that contain a -NH2 and a -NO radical. Many members of this group have carcinogenic and mutagenic properties. Nitrosamine
D002621 Chemistry A basic science concerned with the composition, structure, and properties of matter; and the reactions that occur between substances and the associated energy exchange.
D005502 Food Substances taken in by the body to provide nourishment. Foods
D005511 Food Handling Any aspect of the operations in the preparation, processing, transport, storage, packaging, wrapping, exposure for sale, service, or delivery of food. Food Processing,Handling, Food,Processing, Food
D005750 Gastric Juice The liquid secretion of the stomach mucosa consisting of hydrochloric acid (GASTRIC ACID); PEPSINOGENS; INTRINSIC FACTOR; GASTRIN; MUCUS; and the bicarbonate ion (BICARBONATES). (From Best & Taylor's Physiological Basis of Medical Practice, 12th ed, p651) Gastric Juices,Juice, Gastric,Juices, Gastric
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000596 Amino Acids Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. Twenty alpha-amino acids are the subunits which are polymerized to form proteins. Amino Acid,Acid, Amino,Acids, Amino

Related Publications

P J Groenen, and M W de Cock-Bethbeder, and J Bouwman, and J H Dhont
April 1988, Cancer letters,
P J Groenen, and M W de Cock-Bethbeder, and J Bouwman, and J H Dhont
January 1985, Archiv fur Geschwulstforschung,
P J Groenen, and M W de Cock-Bethbeder, and J Bouwman, and J H Dhont
January 1975, Journal of agricultural and food chemistry,
P J Groenen, and M W de Cock-Bethbeder, and J Bouwman, and J H Dhont
October 1986, Environmental science & technology,
P J Groenen, and M W de Cock-Bethbeder, and J Bouwman, and J H Dhont
January 1983, IARC scientific publications,
P J Groenen, and M W de Cock-Bethbeder, and J Bouwman, and J H Dhont
June 1982, Journal of dental research,
P J Groenen, and M W de Cock-Bethbeder, and J Bouwman, and J H Dhont
August 1978, Food and cosmetics toxicology,
P J Groenen, and M W de Cock-Bethbeder, and J Bouwman, and J H Dhont
March 1988, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association,
P J Groenen, and M W de Cock-Bethbeder, and J Bouwman, and J H Dhont
August 1987, Cancer letters,
P J Groenen, and M W de Cock-Bethbeder, and J Bouwman, and J H Dhont
September 2023, Scientific reports,
Copied contents to your clipboard!