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Human Raji lymphoblast-like cells were propagated in the presence of various concentrations of N-methyl-N-nitrosourea (MNU) and the degree of enzymatic methylation of newly synthesized DNA was analysed by two independent methods. The overall extent of enzymatic DNA methylation was measured on the basis of [14C]deoxycytidine derived radioactivity incorporated into DNA 5-methylcytosine and cytosine residues. Enzymatic methylation of internal cytosines at 5'-CCGG-3' sequences of Raji DNA was analysed by use of the bacterial restriction enzyme HpaII and its isoschizomer MspI. The data obtained by both methods indicate that the treatment with MNU causes a lower level of enzymatic methylation of newly synthesized DNA. This lower extent of DNA methylation persists in the absence of the carcinogen in the cell cycles following the treatment.
T L Boehm, and
D Drahovsky
April 1980,
Bollettino della Societa italiana di biologia sperimentale,
T L Boehm, and
D Drahovsky
May 1978,
Bollettino della Societa italiana di biologia sperimentale,
T L Boehm, and
D Drahovsky
July 1976,
Nucleic acids research,
T L Boehm, and
D Drahovsky
October 1977,
Radiation and environmental biophysics,
T L Boehm, and
D Drahovsky
November 1988,
Mutation research,
T L Boehm, and
D Drahovsky
September 1983,
Chemico-biological interactions,
T L Boehm, and
D Drahovsky
February 1990,
Carcinogenesis,
T L Boehm, and
D Drahovsky
June 1979,
Biochimica et biophysica acta,
T L Boehm, and
D Drahovsky
May 1973,
FEBS letters,
T L Boehm, and
D Drahovsky
February 1984,
Biochimica et biophysica acta,
