Salmonella isolation with Rappaport's enrichment medium of different compositions. 1981

P Vassiliadis, and D Trichopoulos, and J Papadakis, and V Kalapothaki, and X Zavitsanos, and C Serie

One hundred samples of feces of normal pigs were examined for the presence of salmonellae. The feces were pre-enriched for 18 h in peptone water and then, 0.1 ml of the pre-enrichment medium was inoculated in 10 ml of the original Rappaport medium (R30), and two modifications (R25 and R10) of this medium. Two of the broths (R30 and R25) were incubated at 37 degrees C (R30/37 degrees C and R25/37 degrees C) while the R10 medium was incubated at 43 degrees C (R10/43 degrees C). In addition 100 ml of R10 medium (R10/100 ml/43 degrees C) and 100 ml of the standardized Muller Kauffmann's tetrathionate broth (MK/100 ml/43 degrees C) (Edel and Kampelmacher, 1969) were inoculated with 1 ml and 10 ml, respectively, of the pre-enrichment medium, and incubated at 43 degrees C. With the method R10/100 ml/43 degrees C, 37 samples were found positive while with the method MK/100 ml/43 degrees C only 22 positive samples were detected (P less than 0.001). With the Rappaport media in 10 ml volumes, the modification R10 yielded 26 positive samples, while the R30 and R25 broths yielded only 15 and 19 positive samples. All Rappaport procedures had a much stronger inhibition of the competing organisms, particularly those giving Salmonella-like colonies (lactose and sucrose negative), than the MK/100 ml/43 degrees C method, a fact of considerable importance in everyday's practice.

UI MeSH Term Description Entries
D003470 Culture Media Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN. Media, Culture
D005243 Feces Excrement from the INTESTINES, containing unabsorbed solids, waste products, secretions, and BACTERIA of the DIGESTIVE SYSTEM.
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D012475 Salmonella A genus of gram-negative, facultatively anaerobic, rod-shaped bacteria that utilizes citrate as a sole carbon source. It is pathogenic for humans, causing enteric fevers, gastroenteritis, and bacteremia. Food poisoning is the most common clinical manifestation. Organisms within this genus are separated on the basis of antigenic characteristics, sugar fermentation patterns, and bacteriophage susceptibility.
D013552 Swine Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA). Phacochoerus,Pigs,Suidae,Warthogs,Wart Hogs,Hog, Wart,Hogs, Wart,Wart Hog
D013696 Temperature The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms. Temperatures

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